Recombinant Rhesus Macaque IL-3 Protein

Name: Recombinant Rhesus Macaque IL-3 Protein
Brand: Novus Biologicals
SKU: NBP2-35308

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35308

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Product Datasheet / COA

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Availability

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 124 amino acids corresponding to IL-3 Source: E. coli

Uniprot ID: P25140

Amino Acid Sequence: APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-3 as determined by LAL method.

Predicted Molecular Mass

14.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-3 protein is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2 x 10^5 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rhesus Macaque IL-3 Protein

HPLC: Recombinant Rhesus Macaque IL-3 Protein [NBP2-35308]

HPLC: Recombinant Primate IL-3 Protein [NBP2-35308] - Primate IL3 Protein [NBP2-35308]

Formulation, Preparation and Storage

NBP2-35308

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-3

Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The rhesus macaque IL-3 reported to be a monomer, as it is known, contains 124 amino acids residues which is a single non-glycosylated polypeptide. Specifically, recombinant rhesus macaque, human and murine IL-3 share low homology.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Gene Symbol

IL3

Additional IL-3 Products

Product Documents for Recombinant Rhesus Macaque IL-3 Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Rhesus Macaque IL-3 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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