Recombinant Rat IL-4 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35252
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 125 amino acids corresponding to IL-4 Source: E. coli
Uniprot ID: P20096
Amino Acid Sequence: HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IL-4 as determined by LAL method.
Predicted Molecular Mass
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL4 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat splenocytes is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.
Protein / Peptide Type
Recombinant Protein
Reviewed Applications
Read 1 review rated 5 using NBP2-35252 in the following applications:
Scientific Data Images for Recombinant Rat IL-4 Protein
![SDS-PAGE: Recombinant Rat IL-4 Protein [NBP2-35252]](https://resources.bio-techne.com/images/products/Rat-IL-4-Protein-SDS-Page-NBP2-35252-img0001.jpg "SDS-PAGE: Recombinant Rat IL-4 Protein [NBP2-35252]")
SDS-PAGE: Recombinant Rat IL-4 Protein [NBP2-35252]
SDS-Page: Rat IL-4 Protein [NBP2-35252]
Bioactivity: Recombinant Rat IL-4 Protein [NBP2-35252] - Gene expression of macrophages. Culturing with IL-4 increased Arg1 without affecting iNOS or IL-6. Rat macrophages were cultured with M-CSF. After maturation (7 days) in vitro, macrophages were cultured with with IL-4 + M-CSF or M-CSF alone. Changes in gene expression were assayed using RT-PCR using Image from verified customer review.
Formulation, Preparation and Storage
NBP2-35252
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 5% trehalose. |
| Preservative | 5% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-4
Long Name
Interleukin 4
Alternate Names
BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12
Gene Symbol
IL4
UniProt
Additional IL-4 Products
Product Documents for Recombinant Rat IL-4 Protein
Product Specific Notices for Recombinant Rat IL-4 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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