Recombinant Rat Progranulin/PGRN FLAG Protein

Name: Recombinant Rat Progranulin/PGRN FLAG Protein
Brand: Novus Biologicals
SKU: NBP3-18147

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18147

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

HEK293

Accession #

NP_058809

Tag

FLAG

Conjugate

Unconjugated

Applications

SDS-PAGE, Western Blot

View all Progranulin/PGRN Proteins and Enzymes »

Product Specifications

Description

Signal peptide and rat progranulin (aa 1-602) are fused at the C-terminus to a FLAG®-tag.

Source: HEK 293 cells

NCBI: NP_058809

Purity

>95% pure by SDS-PAGE

Endotoxin Level

<0.1EU/ug purified protein (LAL test; Lonza).

Predicted Molecular Mass

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat Progranulin/PGRN FLAG Protein

Western Blot: Recombinant Rat Progranulin/PGRN FLAG Protein [NBP3-18147]

Western Blot: Recombinant Rat Progranulin/PGRN FLAG Protein [NBP3-18147] - To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.

Formulation, Preparation and Storage

NBP3-18147

Formulation	Lyophilized from 0.2um-filtered solution in PBS
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Progranulin/PGRN

Progranulin, also known as Acrogranin, PCDGF, and Epithelin/Granulin Precursor, is a widely expressed glycoprotein that can undergo proteolysis to generate numerous Granulin peptides. Progranulin plays a role in the regulation of mesothelial differentiation, macrophage development, sexual differentiation of the brain, cartilage proliferation, wound repair, and the development of rheumatoid arthritis and osteoarthritis. It is often upregulated in cancer and contributes to tumor progression. Progranulin upregulation or mutation is associated with several neurological disorders including frontotemporal dementia, Creutzfeldt-Jakob disease, Alzheimer’s disease, and motor neuron disease.

Alternate Names

Acrogranin, GEP, GP88, GRN, PCDGF, PEPI, PGRN, Proepithelin

Gene Symbol

GRN

UniProt

NP_058809 (Rat)

Additional Progranulin/PGRN Products

Product Documents for Recombinant Rat Progranulin/PGRN FLAG Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Rat Progranulin/PGRN FLAG Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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