Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10504-DC

R&D Systems, part of Bio-Techne
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10504-DC-050
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Key Product Details

Source

CHO

Accession #

XP_015296329.1

Conjugate

Unconjugated

Applications

Bioactivity

View all DC-SIGN/CD209 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey DC-SIGN/CD209 protein

Hemagglutinin Tag
(YPYDVPDYA)

 Cynomolgus Monkey DC-SIGN/CD209
(Lys62-Glu406)
Accession # XP_015296329.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr of HA tag

Predicted Molecular Mass

40 kDa

SDS-PAGE

44-54 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells.
The ED50 for this effect is 1.25-8 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 10504-DC in the following applications:

Scientific Data Images for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag Protein Bioactivity.

Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (Catalog # 10504-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 1.25-8 µg/mL.

Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (10504-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-54 kDa.

Formulation, Preparation and Storage

10504-DC
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: DC-SIGN/CD209

Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 40 kDa, 344 amino acid (aa) type II transmembrane protein, with the extracellular domain (ECD) containing a Ca2+-dependent carbohydrate-binding lectin domain (2). Within the ECD, cynomolgus DC-SIGN/CD209 shares 85% sequence identity with human DC-SIGN/CD209. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self-glycoproteins including ICAMs (2, 3). DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (4,5).

References

  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  4. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  5. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

Alternate Names

CD209, CLEC4L, DC-SIGN1, DCSIGN

Entrez Gene IDs

30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)

Gene Symbol

CD209

UniProt

XP_015296329.1

Additional DC-SIGN/CD209 Products

Product Documents for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

For research use only

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