Recombinant Cynomolgus Fc gamma RIII/CD16 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9224-FC
Key Product Details
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey Fc gamma RIII (CD16) protein
Gly17-Gln208, with a C-terminal 6-His tag
Gly17-Gln208, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly17, Glu21 and Met18
Predicted Molecular Mass
23 kDa
SDS-PAGE
36-44 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey Fc gamma RIII (CD16) was immobilize on a His Tag antibody coated plate, it binds biotinylated Human IgG. The concentration of biotinylated Human IgG that produces 50% of the optimal binding response is approximately
0.15-0.75 μg/mL.
When Recombinant Cynomolgus Monkey Fc gamma RIII (CD16) was immobilize on a His Tag antibody coated plate, it binds biotinylated Human IgG. The concentration of biotinylated Human IgG that produces 50% of the optimal binding response is approximately
0.15-0.75 μg/mL.
Scientific Data Images for Recombinant Cynomolgus Fc gamma RIII/CD16 Protein, CF
Recombinant Cynomolgus Fc gamma RIII/CD16 Protein Binding Activity
When Recombinant Cynomolgus Fc gamma RIII (CD16) (Catalog # 9224-FC) was immobilized on a His Tag antibody (Catalog # MAB050) coated plate, it binds Biotinylated Human IgG with an ED50 of 0.15-0.75 µg/mL.Formulation, Preparation and Storage
9224-FC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 200 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Fc gamma RIII (CD16)
Ig-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain (4). In humans, Fc gamma RIIIA/CD16a is expressed as a 50-70 kDa transmembrane activating receptor on NK cells, T cells, monocytes, and macrophages (2). It is closely related to the GPI-linked Fc gamma RIIIB which is expressed on human neutrophils and eosinophils (1, 3). These two proteins share 97% amino acid (aa) identity within their extracellular domains (ECD) (5). Within the ECD, mature cynomolgus Fc gamma RIII shares 92% and 90% aa sequence identity with human Fc gamma RIIIA and Fc gamma RIIIB, respectively. Fc gamma RIII surface expression requires interaction with an accessory chain, either the common gamma-chain or CD3 zeta (8, 9). Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA (10). Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity (11-14). Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis (14). Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases (12, 13). Cynomolgus Fc gamma RIII binds to cynomolgus IgG subclasses 1-4, to human IgG1 and 3, and more weakly to human IgG2 and 4 (15).
References
- Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
- Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
- Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
- Rogers, K.A. et al. (2008) J. Immunol. 177:3848.
- Scallon, B.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5079.
- Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
- Dall'Ozzo, S. et al. (2004) Cancer Res. 64:4664.
- Kim, M.-K. et al. (2003) Blood 101:4479.
- Lanier, L.L. et al. (1989) Nature 342:803.
- Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
- Li, P. et al. (2007) J. Biol. Chem. 282:6210.
- Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
- Masuda, M. et al. (2006) Atherosclerosis 188:377.
- Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.
- Warncke, M. et al. (2012) J. Immunol. 188:4405.
Long Name
Fc gamma Receptor III
Alternate Names
FcgRIII
Gene Symbol
FCGR3
UniProt
Additional Fc gamma RIII (CD16) Products
Product Documents for Recombinant Cynomolgus Fc gamma RIII/CD16 Protein, CF
Product Specific Notices for Recombinant Cynomolgus Fc gamma RIII/CD16 Protein, CF
For research use only
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