Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF

R&D Systems, part of Bio-Techne | Catalog # 10215-CL

GS Linker, Defined heterodimer stoichiometry.
R&D Systems, part of Bio-Techne
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10215-CL-050
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Key Product Details

Source

HEK293

Accession #

NP_001274204 (IL-12p40) & XP_005546300 (IL-12p35)

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-12 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-12 protein
Cynomolgus Monkey IL-12p40
(Ile23-Ser328)
Accession # NP_001274204		 GGGSGGGSGGGS  Cynomolgus Monkey IL-12p35
(Arg57-Ser253)Accession # XP_005546300
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile23

Predicted Molecular Mass

58 kDa

SDS-PAGE

65-78 kDa, under reducing conditions

Activity

Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells.
The ED50 for this effect is 0.15-0.9 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF

Recombinant Cynomolgus Monkey IL-12 (linked heterodimer) Bioactivity

Recombinant Cynomolgus Monkey IL-12 (linked heterodimer) Bioactivity

Recombinant Cynomolgus Monkey IL-12 (Catalog # 10215-CL) enhances IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 0.15-0.9 ng/mL.
Recombinant Cynomolgus Monkey IL-12 (linked heterodimer) SDS-PAGE

Recombinant Cynomolgus Monkey IL-12 (linked heterodimer) SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey IL-12 (10215-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-78 kDa.

Formulation, Preparation and Storage

10215-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit (1, 2). The cynomolgus monkey p35 subunit shares 93.4% sequence identity with the human homolog, and the p40 subunit shares 96.4% identity with its human homolog. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation (2, 3). Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent (1-3).

References

  1. Hasegawa, H. et al. (2016) Front. Immunol. 7:479.
  2. Schurich, A. et al. (2017) Rheumatology. 57:246.
  3. Agarwal, P. (2009). J Immunol. 183:1695.

Long Name

Interleukin 12

Alternate Names

CLMF1, IL12, IL12A, NKSF1

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Gene Symbol

IL12A

UniProt

NP_001274204 (IL-12p40) & XP_005546300 (IL-12p35)

Additional IL-12 Products

Product Documents for Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF

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Product Specific Notices for Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF

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