Recombinant Cynomolgus Monkey Tie-2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10376-T2

R&D Systems, part of Bio-Techne
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10376-T2-100
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Key Product Details

Source

HEK293

Accession #

XP_005581645.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Tie-2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey Tie-2 protein
Cynomolgus Monkey Tie-2
(Ala23-Lys745)
Accession # XP_005581645.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

107 kDa

SDS-PAGE

118-132 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey Tie-2 Fc Chimera (Catalog # 10376-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds with an ED50 of 1.5-15 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey Tie-2 Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey Tie-2 Fc Chimera Protein Binding Activity

Recombinant Cynomolgus Monkey Tie-2 Fc Chimera Protein Binding Activity

When Recombinant Cynomolgus Monkey Tie-2 Fc Chimera (Catalog # 10376-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Aniopoietin-2 (Catalog # 623-AN) binds with an ED50 0f 1.5-15 ng/mL.
Recombinant Cynomolgus Monkey Tie-2 Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus Monkey Tie-2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Tie-2 Fc Chimera (Catalog # 10376-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 118-132 kDa and 220 - 250 kDa, respectively.

Formulation, Preparation and Storage

10376-T2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region (1). These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis (2). Cynomolgus Monkey Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with a 22 residue putative signal peptide, a 723 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects (3).

References

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
  3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Alternate Names

CD202b, TEK, Tie2

Entrez Gene IDs

7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)

Gene Symbol

TEK

UniProt

XP_005581645.1

Additional Tie-2 Products

Product Documents for Recombinant Cynomolgus Monkey Tie-2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Cynomolgus Monkey Tie-2 Fc Chimera Protein, CF

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