Recombinant Cynomolgus/Rhesus Tyro3/Dtk Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11266-DT

R&D Systems, part of Bio-Techne
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11266-DT-100
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Key Product Details

Source

CHO

Accession #

XP_005559306.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Tyro3/Dtk Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived Tyro3/Dtk protein
Cynomolgus/Rhesus Tyro3/Dtk
(Ala41-Ser428)
Accession # XP_005559306.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala41

Predicted Molecular Mass

68 kDa

SDS-PAGE

92-102 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey/Rhesus Macaque Tyro3/Dtk Fc Chimera (Catalog # 11266-DT) is immobilized at 0.500 µg/mL (100 µL/well), Recombinant Human Gas6 Protein  (Catalog # 885-GSB) binds with an ED50 of 50.0-400 ng/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus Tyro3/Dtk Fc Protein, CF

Recombinant Cynomolgus Monkey/Rhesus Macaque Tyro3/Dtk Fc Chimera Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey/Rhesus Macaque Tyro3/Dtk Fc Chimera Protein (Catalog # 11266-DT) is immobilized at 0.500 µg/mL (100 µL/well), Recombinant Human Gas6 Protein (885-GSB) binds with an ED50 of 50.0-400 ng/mL.

Recombinant Cynomolgus Monkey/Rhesus Macaque Tyro3/Dtk Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque Tyro3/Dtk Fc Chimera Protein (Catalog # 11266-DT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 92-102 kDa and 180-200 kDa, respectively.

Formulation, Preparation and Storage

11266-DT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily (1, 2). The overall genomic structure of Dtk is virtually identical to that determined for the human UFO gene. This particular genomic organization is likely to have been duplicated and closely maintained throughout evolution (3). The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases (1). All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anti-coagulation factor protein S (1, 2). The binding affinities for Gas6 is in the order of Axl > Dtk > Mer (1). Gas6 binding induces tyrosine phosphorylation and down-stream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (1, 2). Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain (4). Within the ECD, cynomolgus monkey/rhesus macaque DTK shares 99% sequence identity with human DTK.

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
  3. Paula, M. et al. (1996) Genomics 31:13.
  4. Philip, S. et al. (1994) Growth Factor 11:125.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human); 22174 (Mouse); 102135978 (Cynomolgus Monkey)

Gene Symbol

TYRO3

UniProt

XP_005559306.1

Additional Tyro3/Dtk Products

Product Documents for Recombinant Cynomolgus/Rhesus Tyro3/Dtk Fc Protein, CF

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Product Specific Notices for Recombinant Cynomolgus/Rhesus Tyro3/Dtk Fc Protein, CF

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