Recombinant Human Gas6 Protein, CF Best Seller
R&D Systems, part of Bio-Techne | Catalog # 885-GSB
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Gas6 protein
Ala49-Ala678, with a C-terminal 6-His tag
Ala49-Ala678, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala49
Predicted Molecular Mass
70.5 kDa
SDS-PAGE
70-85 kDa, reducing conditions
Activity
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703.
The ED50 for this effect is 25-150 ng/mL
The ED50 for this effect is 25-150 ng/mL
Reviewed Applications
Read 7 reviews rated 4.4 using 885-GSB in the following applications:
Scientific Data Images for Recombinant Human Gas6 Protein, CF
Bioactivity of Recombinant Human Gas6 protein
Recombinant Human Gas6 885-GSB down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 25-150 ng/mL.Formulation, Preparation and Storage
885-GSB
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Citrate. |
| Reconstitution |
Reconstitute at 500 μg/mL in sterile water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Gas6
EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma-carboxylation of the Gla domain (12, 16).
References
- Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
- Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
- Stitt, T.N. et al. (1995) Cell 80:661.
- Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
- Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
- Goruppi, S. et al. (1997) FEBS Lett. 415:59.
- Sather, S. et al. (2007) Blood 109:1026.
- Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
- Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
- Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
- Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
- Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
- Gallicchio, M. et al. (2005) Blood 105:1970.
- Caraux, A. et al. (2006) Nat. Immunol. 7:747.
- Wu, Y. et al. (2005) J. Cell Sci. 118:539.
- Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
Long Name
Growth-arrest-specific Protein 6
Alternate Names
AXLLG
Gene Symbol
GAS6
UniProt
Additional Gas6 Products
Product Documents for Recombinant Human Gas6 Protein, CF
Product Specific Notices for Recombinant Human Gas6 Protein, CF
For research use only
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