Recombinant Feline CD8 alpha Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9649-CD

R&D Systems, part of Bio-Techne
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9649-CD-050
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Key Product Details

Source

NS0

Accession #

P41688

Conjugate

Unconjugated

View all CD8 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived feline CD8 protein
Ala22-Tyr188, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

19 kDa

SDS-PAGE

28-31 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9649-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8 is a dimeric complex made up of two Ig superfamily members (1). The CD8 alpha chain is a type I transmembrane glycoprotein that can dimerize with either itself forming CD8 alpha alpha, or with a 30-35 kDa CD8 beta chain, forming CD8 alpha beta (2). CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR co-receptor, while CD8 alpha alpha promotes T cell survival and differentiation (1, 3). The mature feline CD alpha consists of a 167 amino acid (aa) extracellular domain (ECD), and runs at ~30 kDa on reduced SDS-PAGE. Within the ECD, feline CD8 alpha shares 58%, 47%, 51%, and 69% amino acid sequence identity with human, mouse, porcine, and canine CD8 alpha, respectively.

References

  1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
  2. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
  3. Cole, D.K. et al. (2012) Immunology 137:139.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

P41688

Additional CD8 Products

Product Documents for Recombinant Feline CD8 alpha Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Feline CD8 alpha Protein, CF

For research use only

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