Recombinant Human Active PYK2/FAK2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4589-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human PYK2/FAK2 protein
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal His tag
SDS-PAGE
39 kDa
Activity
The specific activity of PYK2 is typically 216-292 nmol/min/mg using a poly (Glu:Tyr, 4:1) synthetic peptide substrate.
Scientific Data Images for Recombinant Human Active PYK2/FAK2 Protein, CF
Recombinant Human Active PYK2/FAK2 Protein SDS-PAGE.
The approximate molecular weight is 39 kDa and the purity is > 95%.Formulation, Preparation and Storage
4589-KS
| Formulation | Supplied in 50 mM Sodium Phosphate (pH 7.0), 300 mM NaCl, 0.25 mM DTT, 150 mM Imidazole, 0.1 mM PMSF, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: PYK2/FAK2
PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family. PYK2/FAK2 can be activated by a variety of extracellular signals that elevate intracellular calcium concentration and by stress signals (1). Unlike FAK, which is widely expressed in various tissues and links transmembrane integrin receptors to intracellular pathways, PYK2/FAK2 is expressed mainly in the central nervous system and in cells derived from hematopoietic lineages. In osteoclasts, although FAK is expressed, PYK2/FAK2 appears to be the predominant mediator of integrin alphaV beta3 signaling events that influence osteoclast physiology and pathology (2).
References
- Avraham, H. et al. (2000) Cell Signal. 12:123.
- Xiong, W.C. et al. (2003) Front. Biosci. 8:1219.
Long Name
Proline-rich Tyrosine Kinase 2
Alternate Names
CADTK, CAKB, FADK2, FAK2, FRNK, PTK2B, RAFTK
Gene Symbol
PTK2B
UniProt
Additional PYK2/FAK2 Products
Product Documents for Recombinant Human Active PYK2/FAK2 Protein, CF
Product Specific Notices for Recombinant Human Active PYK2/FAK2 Protein, CF
For research use only
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