Recombinant Human Angiopoietin-like 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9746-AN/CF

R&D Systems, part of Bio-Techne
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9746-AN-025/CF
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Key Product Details

Source

NS0

Accession #

Q9UKU9.1

Structure / Form

Disulfide-linked homooligomer

Conjugate

Unconjugated

Applications

Bioactivity

View all Angiopoietin-like Protein 2/ANGPTL2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 2/ANGPTL2 protein
Gln23-His493, with a C-terminal 9-His tag

Purity

>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln23 inferred from enzymatic pyroglutamate treatment revealing Glu24

Predicted Molecular Mass

56 kDa

SDS-PAGE

60-69 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Angiopoietin‑like Protein 2/ANGPTL2 is coated at 2 μg/mL, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078‑T4) binds with an ED50 of 0.02‑0.12 μg/mL

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL).
The ED50 for this effect is 0.3-1.8 μg/mL.

Scientific Data Images for Recombinant Human Angiopoietin-like 2 Protein, CF

Recombinant Human Angiopoietin-like 2 Protein Binding Activity

Recombinant Human Angiopoietin-like 2 Protein Binding Activity

When Recombinant Angiopoietin-like Protein 2/ANGPTL2 (Catalog # 9746-AN) is coated at 2 µg/mL, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 0.02-0.12 µg/mL.

Formulation, Preparation and Storage

9746-AN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS with Trehalose.
Reconstitution
Reconstitute at 200 μg/mL in water.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2, is a secreted 56 kDa glycoprotein that contains a coiled coil domain and a fibrinogen like domain (1). ANGPTL2 is one of the eight members of the Angiopoietin like family proteins, which are structurally similar to angiopoietins (2). Mature human ANGPTL2 shares 89% sequence identity with both mouse and rat homologs. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6, 7). ANGPTL2 functions as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled-coil domain (8). Receptor of ANGPTL2 is recently identified to be LILRB2, and both the coiled-coil domain and fibrinogen like domain are required for optimal binding (9).

References

  1. Kim, I. et al. (1999), J Biol Chem 274:26523.
  2. Santulli, G. et al. (2014) Front Endocrinol 5:4.
  3. Tabata, M. et al. (2009) Cell Metab 10:178.
  4. Kubota, Y. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:13502.
  5. Kikuchi, R. et al. (2008) Cancer Res 68:5067.
  6. Odagiri, H. et al. (2014) Sci Signal 7:ra7.
  7. Endo, M. et al. (2012) Cancer Res 72:1784.
  8. Broxmeyer, H.E. et al. (2012) Blood Cells Mol Dis 48:25.
  9. Deng, M. et al. (2014) Blood 124:924.

Alternate Names

ANGPTL2, ANGRP2

Entrez Gene IDs

23452 (Human); 26360 (Mouse)

Gene Symbol

ANGPTL2

UniProt

Q9UKU9.1

Additional Angiopoietin-like Protein 2/ANGPTL2 Products

Product Documents for Recombinant Human Angiopoietin-like 2 Protein, CF

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