Recombinant Mouse Thrombopoietin Protein

R&D Systems, part of Bio-Techne | Catalog # 488-TO

R&D Systems, part of Bio-Techne
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Carrier Free
488-TO-005/CF
488-TO-025/CF
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488-TO-200/CF
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With Carrier
488-TO-005
488-TO-025
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488-TO-200
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Key Product Details

Source

NS0

Accession #

P40226

Conjugate

Unconjugated

Applications

Bioactivity

View all Thrombopoietin/Tpo Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Thrombopoietin/Tpo protein
Ser22-Thr356

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

35 kDa

SDS-PAGE

80-90 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 0.4-2.4 ng/mL.

Scientific Data Images for Recombinant Mouse Thrombopoietin Protein

Recombinant Mouse Thrombopoietin Protein Bioactivity

Recombinant Mouse Thrombopoietin Protein Bioactivity

Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.4-2.4 ng/mL.
Recombinant Mouse Thrombopoietin Protein SDS-PAGE

Recombinant Mouse Thrombopoietin Protein SDS-PAGE

1 μg/lane of Recombinant Mouse Thrombopoietin/Tpo was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 80-90 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 488-TO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 488-TO/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin/Tpo

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1-3). The 356 amino acid (aa) mouse Tpo precursor is cleaved to yield the 335 aa mature protein. Mature mouse Tpo shares 71% and 81% aa sequence homology with human and rat Tpo, respectively. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of mouse Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10-13). It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling (15, 16).

References

  1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.

  2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.

  3. Li, J. et al. (1999) Br. J. Haematol. 106:345.

  4. Bartley, T.D. et al. (1994) Cell 77:1117.

  5. de Sauvage, F.J. et al. (1994) Nature 369:533.

  6. Marcucci, R. and M. Romano  (2008) Biochim. Biophys. Acta 1782:427.

  7. Kaushansky, K. et al. (1994) Nature 369:568.

  8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.

  9. Broudy, V.C. et al. (1995) Blood 85:1719.

  10. Lok, S.I. et al. (1994) Nature 369:565.

  11. Chen, J. et al. (1995) Blood 86:4054.

  12. Oda, A. et al. (1996) Blood 87:4664.

  13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.

  14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.

  15. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.

  16. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.

Alternate Names

MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Gene Symbol

THPO

UniProt

P40226

Additional Thrombopoietin/Tpo Products

Product Documents for Recombinant Mouse Thrombopoietin Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 488-TO (with carrier)
Download SDS 488-TO/CF (carrier free)

Product Specific Notices for Recombinant Mouse Thrombopoietin Protein

For research use only

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