Recombinant Human Axl Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11011

R&D Systems, part of Bio-Techne
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AVI11011-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P30530.4

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Axl Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Axl protein
Human Axl
(Ala26 - Trp450)
Accession # P30530.4		 IEGRMD Human IgG1 Fc
(Pro100 - Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala26, Glu33

Predicted Molecular Mass

74 kDa

SDS-PAGE

89 - 115 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Gas6 (Catalog # 885-GSB) is immobilized at 2.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Axl Fc Chimera Avi-tag binds with an ED50 of 2.50-15.0 ng/mL.

Scientific Data Images for Recombinant Human Axl Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human Axl Fc Chimera Avi-tag Protein Binding Activity.

When recombinant human Gas6 (885-GSB) is immobilized at 2.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Axl Fc Chimera Avitag Protein (Catalog # AVI11011) binds with an ED50 of 2.50-15.0 ng/mL.

Biotinylated Recombinant Human Axl Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Axl Fc Chimera Avi-tag Protein (Catalog # AVI11011) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 89-115 kDa and 180-230 kDa, respectively.

Formulation, Preparation and Storage

AVI11011
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature human Axl consists of a 426 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2). Within the ECD, human Axl shares approximately 82% aa sequence identity with mouse and rat Axl. A short alternately spliced form of human Axl has a 9 aa deletion in the extracellular juxtamembrane region (2). Axl binds the vitamin K-dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (3). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (4), macrophage and dendritic cell phagocytosis of apoptotic cells (5), NK cell development from hematopoietic progenitor cells (6), and in vivo angiogenesis (7). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (7, 8). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (9). It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses (10). A 70-80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (11, 12). Our Avi-tag Biotinylated Axl Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35.
  2. O’Bryan, J.P. et al. (1991) Mol. Cell. Biol. 11:5016.
  3. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  4. Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797.
  5. Seitz, H.M. et al. (2007) J. Immunol. 178:5635.
  6. Park, I.-K. et al. (2009) Blood 113:2470.
  7. Holland, S. et al. (2005) Cancer Res. 65:9294.
  8. Rankin, E.B. et al. (2010) Cancer Res. 70:7570.
  9. Korshunov, V.A. et al. (2006) Circ. Res. 98:1446.
  10. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
  11. O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551.
  12. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.

Long Name

Axl Receptor Tyrosine Kinase

Alternate Names

AI323647, ARK, JTK11, Tyro7, UFO

Entrez Gene IDs

558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)

Gene Symbol

AXL

UniProt

P30530.4

Additional Axl Products

Product Documents for Recombinant Human Axl Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human Axl Fc Chimera Avi-tag Protein, CF

For research use only

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