Recombinant Human Bcl-2 related protein A1 (aa 1-152), CF
R&D Systems, part of Bio-Techne | Catalog # 1160-A1
Key Product Details
Source
E. coli
Accession #
Structure / Form
Monomer
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
E. coli-derived human Bcl-2 related protein A1 protein
Thr2-Ser152, with a C-terminal 6-His tag
Thr2-Ser152, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr2
Predicted Molecular Mass
18 kDa
SDS-PAGE
16 kDa, non-reducing conditions
Activity
Measured by its ability to inhibit Caspase-8-cleaved BID-mediated release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog # MCTC0) to quantify cytochrome c.
The typical EC50 for human Bcl‑2 related protein A1 Minus C-terminus in this assay is 15-200 nM.
The typical EC50 for human Bcl‑2 related protein A1 Minus C-terminus in this assay is 15-200 nM.
Formulation, Preparation and Storage
1160-A1
| Formulation | Supplied as a 0.2 μm filtered solution in NaH2PO4, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Bcl-2 related protein A1
Bcl‑2 related protein A1 (BCL2A1) is a member of the Bcl-2 family of proteins that regulate the outer mitochondrial membrane permeability. BCL2A1 is an anti‑apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. BCL2A1 is present on the outer mitochondrial membrane and is also found on other intracellular membranes in some cell types. Natural BCL2A1 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant BCL2A1 is missing the mitochondrial targeting sequence but maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by BCL2A1 appears to occur by binding to the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
References
- Duriez, P. et al. (2000) J. Biol. Chem. 275(24):18099.
- Holmgreen, S. et al. (1999) Cell Death and Diff. 6:525.
- Lin, Ey (1993) J. Immunol. 151:197.
- Karsan, A. (1996) Blood 87:3089.
Alternate Names
BCL2A1, Bcl2L5, Bfl-1, GRS
Gene Symbol
BCL2A1
UniProt
Additional Bcl-2 related protein A1 Products
Product Documents for Recombinant Human Bcl-2 related protein A1 (aa 1-152), CF
Product Specific Notices for Recombinant Human Bcl-2 related protein A1 (aa 1-152), CF
For research use only
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