Recombinant Human BMP-7 Protein Best Seller

R&D Systems, part of Bio-Techne | Catalog # 354-BP

R&D Systems, part of Bio-Techne
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Carrier Free
354-BP-010/CF
354-BP-500/CF
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With Carrier
354-BP-010
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Key Product Details

Source

CHO

Accession #

P18075

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BMP-7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human BMP-7 protein
Ser293-His431

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser293

Predicted Molecular Mass

15.7 kDa (monomer)

SDS-PAGE

18-20 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is 0.1-0.6 µg/mL.

Reviewed Applications

Read 2 reviews rated 4 using 354-BP in the following applications:

Scientific Data Images for Recombinant Human BMP-7 Protein

Graph showing dose-dependent bioactivity of Human BMP-7 protein

Bioactivity of Human BMP-7

Recombinant human BMP-7 (354-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.1-0.6 µg/mL.

Adult Stem Cell-derived Liver Organoids Cultured using Recombinant Human BMP-7 Protein.

Adult stem cell-derived liver organoids were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and liver organoid initiation medium, which includes Recombinant Human BMP-7 (Catalog # 354-BP), Recombinant Human HGF (294-HG), Recombinant Human EGF (236-EG), Recombinant Human FGF-10 (345-FG), Recombinant Human Noggin (6057-NG), Recombinant Human R-Spondin 1 (4645-RS), and Recombinant Human Wnt-3a ( 5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human BMP-7 (Catalog # 354-BP), Recombinant Human HGF (294-HG), Recombinant Human EGF (236-EG), and Recombinant Human FGF-19 (969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (AF2400; red) and counterstained with DAPI (5748; blue).

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 354-BP
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 354-BP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100-200 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-7

Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP-7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide-linked growth factor domains (5, 6). Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4, complexes that show increased potency and range of activity compared to BMP-7 homodimers (7-9). The presence of the propeptides in the BMP-7 tetramer does not diminish the bioactivity of the growth factor domains (6). Secreted BMP-7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix Fibrillin (5). BMP-7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13-15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16).

References

  1. Carreira, A.C. et al. (2015) J. Dent. Res. 93:335.
  2. Weiskirchen, R. and S.K. Meurer (2013) Front. Biosci. (Landmark Ed.) 18:1407.
  3. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
  4. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
  5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
  6. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
  7. Israel, D.I. et al. (1996) Growth Factors 13:291.
  8. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  9. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  10. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
  11. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
  12. Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
  13. Zeisberg, M. et al. (2003) Nat. Med. 9:964.
  14. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
  15. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
  16. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.

Long Name

Bone Morphogenetic Protein 7

Alternate Names

BMP7, OP-1

Entrez Gene IDs

655 (Human); 12162 (Mouse); 85272 (Rat)

Gene Symbol

BMP7

UniProt

P18075

Additional BMP-7 Products

Product Documents for Recombinant Human BMP-7 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 354-BP (with carrier)
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Product Specific Notices for Recombinant Human BMP-7 Protein

For research use only

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