Recombinant Human C1qTNF14/C1qL1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9134-TN

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

O75973

Structure / Form

Noncovalently-linked homotrimer

Conjugate

Unconjugated

Applications

Bioactivity

View all C1qL1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human C1qL1 protein
Thr125-Asp258, with an N-terminal Met and 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

13 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human C1qTNF14/C1qL1 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI3 (Catalog # 9106-BA) that produces 50% of the optimal binding response is approximately 0.4-2.4 μg/mL

Scientific Data Images for Recombinant Human C1qTNF14/C1qL1 Protein, CF

Recombinant Human C1qTNF14/C1qL1 Protein Bioactivity

Recombinant Human C1qTNF14/C1qL1 Protein Bioactivity

When Recombinant Human C1qTNF14/C1qL1 (Catalog # 9134-TN) is coated at 1 µg/mL (100 µL/well), Recombinant Human BAI3 (Catalog # (Catalog # 9106-BA) binds with a typical ED50 of 0.4-2.4 µg/mL.

Formulation, Preparation and Storage

9134-TN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Reconstitution
Reconstitute at 1 mg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1qL1

C1qTNF14 (CTRP14), also known as C1qL1, is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Mature human C1qTNF14 contains a collagen-like region and one C1q-like domain and can form disulfide-linked heteromers with C1qTNF11/C1qL4 (3, 4). Within the C1q-like domain, human C1qTNF14 shares 100% aa sequence identity with mouse and rat C1qTNF14. C1qTNF14 is expressed in the inferior olive, hippocampus, and cerebral cortex (5). Similarly to C1qTNF13/C1qL3, C1qTNF10/C1qL2, and C1qTNF11/C1qL4, C1qTNF14 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (5-8). C1qTNF14/C1qL1 binding to BAI3 induces the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (5, 7).

References

  1. Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
  2. Yuzaki, M. (2010) Eur. J. Neurosci. 32:191.
  3. Ressl, S. et al. (2015) Structure 23:688.
  4. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
  5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
  6. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
  7. Kakegawa, W. et al. (2015) Neuron 85:316.
  8. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.

Long Name

Complement Component 1, Q Subcomponent-Like 1

Alternate Names

C1QRF, C1qTNF14, CTRP14

Entrez Gene IDs

10882 (Human); 23829 (Mouse); 363686 (Rat)

Gene Symbol

C1QL1

UniProt

O75973

Additional C1qL1 Products

Product Documents for Recombinant Human C1qTNF14/C1qL1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human C1qTNF14/C1qL1 Protein, CF

For research use only

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