Recombinant Human CCL26/Eotaxin-3 Protein
R&D Systems, part of Bio-Techne | Catalog # 653-E3
Key Product Details
Product Specifications
Source
E. coli-derived human CCL26/Eotaxin-3 protein
Ser27-Leu94
Ser27-Leu94
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser27
Predicted Molecular Mass
8.2 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CCR3.
The ED50 for this effect is 0.3-1.5 µg/mL.
The ED50 for this effect is 0.3-1.5 µg/mL.
Reviewed Applications
Read 1 review rated 5 using 653-E3 in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 653-E3
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 653-E3/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL26/Eotaxin-3
Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin-3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4.
Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. RecombinantEotaxin-3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. RecombinantEotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin-3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3-transfected cell line.
References
- Gou, R-F. et al. (1999) Genomics 58:313.
- Kitamura, M. et al. (1999) J. Biol. Chem. 274:27975.
- Shinkai, A. et al. (1999) J. Immunol. 163:1602.
Alternate Names
Eotaxin-3, Eotaxin3, SCYA26
Entrez Gene IDs
10344 (Human)
Gene Symbol
CCL26
UniProt
Additional CCL26/Eotaxin-3 Products
Product Documents for Recombinant Human CCL26/Eotaxin-3 Protein
Product Specific Notices for Recombinant Human CCL26/Eotaxin-3 Protein
For research use only
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