Recombinant Human Chromogranin A His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10422-CH

R&D Systems, part of Bio-Techne
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10422-CH-050
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Key Product Details

Source

HEK293

Accession #

P10645.7

Conjugate

Unconjugated

Applications

Bioactivity

View all Chromogranin A Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Chromogranin A protein
Leu19-Gly457, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu19

Predicted Molecular Mass

50 kDa

SDS-PAGE

60-80 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human SCG3 His-tag (Catalog # 10374-SC) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Chromogranin A His‑tag (Catalog # 10422-CH) binds with an ED50 of 40‑360 ng/mL.

Scientific Data Images for Recombinant Human Chromogranin A His-tag Protein, CF

Recombinant Human Chromogranin A His-tag Protein Binding Activity

Recombinant Human Chromogranin A His-tag Protein Binding Activity

When Recombinant Human SCG3 His-tag (10374-SC) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Chromogranin A His-tag (10422-CH) binds with an ED50 of 40-360 ng/mL.
Recombinant Human Chromogranin A His-tag Protein SDS-PAGE

Recombinant Human Chromogranin A His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Chromogranin A His-tag Protein (10422-CH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa.

Formulation, Preparation and Storage

10422-CH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chromogranin A

Chromogranin A (CgA), also known as pituitary secretory protein I (SP-I), is a member of the granin family of regulated secretory proteins. CgA shares several protein characteristics common to the granin family: acidic isoelectric point, the capacity to bind calcium ions, the ability to form aggregates and multiple dibasic cleavage sites. Mature human CgA is 439 amino acids (aa) and contains 10 dibasic, proteolytic cleavage sites, capable of yielding several smaller peptides, each displaying a unique function (4). Mature human CgA shares 63% aa sequence identity with mouse and rat CgA. CgA is expressed exclusively in the secretory dense core granules of most normal and neoplastic neuroendocrine cells (1-3). Increased levels of CgA have been detected inpatients with neuroendocrine tumors as well as non-neuroendocrine tumors, hence CgA is an important serological marker for tumor diagnosis and monitoring tumorprogression/regression (5, 6). It has been demonstrated in mouse model that full-length CgA containing its C-terminal region can impair angiogenesis and tumor growth (5). In addition, CgA can bind to Secretogranin III to regulate the biogenesis of secretory granules (7).

References

  1. Bajetta, E. et al. (1999) Cancer 86:858.
  2. Taupenot, L. et al. (2003) N. Engl. J. Med. 348:1134.
  3. Modlin, I.M. et al. (2010) Am. Surg. Oncol. 17:2427.
  4. Marotta, V. et al. (2018) Endocr Relat Cancer 25:R11
  5. Curnis, F. et al. (2016) Oncotarget 7:72716.
  6. Gkolfinopoulos, S. et al. (2017) World J. Methodol. 7:9.
  7. Hosaka, M. et al. (2004) J Biol Chem 279:3627.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Entrez Gene IDs

1113 (Human); 12652 (Mouse); 24258 (Rat)

Gene Symbol

CHGA

UniProt

P10645.7

Additional Chromogranin A Products

Product Documents for Recombinant Human Chromogranin A His-tag Protein, CF

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Product Specific Notices for Recombinant Human Chromogranin A His-tag Protein, CF

For research use only

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