Recombinant Human Common beta Chain His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9960-CB

R&D Systems, part of Bio-Techne
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9960-CB-050
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Key Product Details

Source

NS0

Accession #

P32927-1

Conjugate

Unconjugated

Applications

Bioactivity

View all Common beta Chain Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Common beta Chain protein
Trp17-Trp443, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly23 & Glu25

Predicted Molecular Mass

49 kDa

SDS-PAGE

57-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Common  beta Chain binds Recombinant Human GM‑CSF (Catalog # 215-GM) in the presence of Recombinant Human GM‑CSF R alpha (Catalog # 706-GR). The concentration of Recombinant Human Common beta that produces 50% of the optimal binding response is 20-120 ng/mL.

Scientific Data Images for Recombinant Human Common beta Chain His-tag Protein, CF

Recombinant Human Common beta Chain His-tag Protein Binding Activity

Recombinant Human Common beta Chain His-tag Protein Binding Activity

In the presence of Recombinant Human GM-CSF Ra (Catalog # 706-GR), Recombinant Human Common Chain binds to Recombinant Human GM-CSF (Catalog # 215-GM) with an ED50 of 20-120 ng/mL.
Recombinant Human Common beta Chain His-tag Protein SDS-PAGE

Recombinant Human Common beta Chain His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Common beta Chain was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 57-65 kDa.

Formulation, Preparation and Storage

9960-CB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common beta Chain

The common beta chain (beta c) is also known as GM-CSF R beta (granulocyte-macrophage colony stimulating factor receptor beta subunit), IL-3 R beta, and IL-5 R beta (gene name CSF2RB and designated CD131) (1, 2). It is a 58-61 kDa type I transmembrane protein that associates with the ligand-specific IL-3 R alpha on T cells and other cells, IL-5 R alpha on eosinophils or GM-CSF R alpha on myeloid cells, to form high affinity receptor complexes (1, 2). The 897 amino acid (aa) human beta c contains a 16 aa signal sequence, a 427 aa extracellular domain (ECD) with two fibronectin type III domains, a transmembrane sequence, and a 437 aa cytoplasmic domain (2, 3). Within the ECD, human beta c shares 57-68% aa sequence identity with mouse, rat, equine, porcine, bovine and canine beta c. Complexes of GM-CSF with its specific alpha subunit then bind preformed beta c dimers, creating 2:2:2 hexamers that can combine to a dodecamer (4:4:4) (3). Beta c association and dimerization via the dimerization of the hexamer to the dodecamer is essential for JAK2 activation via GM-CSF signaling which imparts growth and survival signals (3). Except for eosinophils, beta c is primarily involved when rapid production of leukocytes is needed, rather than for developmental or steady-state cell production (1). Beta c also associates with other receptors, forming heteroreceptor complexes that allow beta c complexes to influence the signaling pathways activated by the associated receptor (1). Beta c thus enhances angiogenesis (when associated with VEGF R2/KDR/Flt-1 or beta 1 integrins), cell protection (with Erythropoietin R), and synergistic growth of stem cells (with SCF R/c- kit) (4-9). Defective production of beta c in humans is a cause of pulmonary alveolar proteinosis (10).

References

  1. Lopez, A.F. et al. (2010) IUBMB Life 62:509.
  2. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
  3. Hansen, G. et al. (2008) Cell 134:496.
  4. Blake, T.J. et al. (2002) J. Leukoc. Biol. 72:1246.
  5. Brines, M. et al. (2004) Proc. Natl. Acad. Sci. USA 101:14907.
  6. Dentelli, P. et al. (2005) Oncogene 24:6394.
  7. Saulle, E. et al. (2009) Br. J. Haematol. 145:399.
  8. Lennartsson, J. et al. (2004) J. Biol. Chem. 279:44544.
  9. Uberti, B. et al. (2010) Oncogene 29:6581.
  10. Dirksen, U. et al. (1997) J. Clin. Invest. 100:2211.

Alternate Names

CD131, CSF2RB, GM-CSF R beta, IL-3 R beta, IL-5 R beta

Entrez Gene IDs

1439 (Human); 12983 (Mouse); 102143353 (Cynomolgus Monkey)

Gene Symbol

CSF2RB

UniProt

P32927-1

Additional Common beta Chain Products

Product Documents for Recombinant Human Common beta Chain His-tag Protein, CF

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Product Specific Notices for Recombinant Human Common beta Chain His-tag Protein, CF

For research use only

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