Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9298

Biotinylated
R&D Systems, part of Bio-Techne
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AVI9298-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Q15762.2

Structure / Form

Biotinylated protein via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all DNAM-1/CD226 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human DNAM-1/CD226 protein
Human DNAM-1
(Glu19-Asn247)
Accession # Q15762.2		 HHHHHH Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu19

Predicted Molecular Mass

29 kDa

SDS-PAGE

35-60 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its binding ability in a functional ELISA.
When 250 ng/mL of Biotinylated Recombinant Human DNAM-1/CD226 His-tag Avi-tag (AVI9298) protein is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human CD155/PVR Fc Chimera  (Catalog # 9174-CD) with an ED50 of 20-100 ng/mL.

Scientific Data Images for Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF

Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein Binding Activity

Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein Binding Activity

When 250 ng/mL of Biotinylated Recombinant Human DNAM-1/CD226 His-tag Avi-tag protein (Catalog # AVI9298) is immobilized onto a Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human CD155/PVR Fc Chimera (Catalog # 9147-CD) with an ED50 of 20-100 ng/mL.
Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein SDS-PAGE

Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human DNAM-1/CD226 His-tag Avi-tag (Catalog # AVI9298) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-60 kDa.

Formulation, Preparation and Storage

AVI9298
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung (11). CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation (12). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 13-15).

References

  1. Zingoni, A. et al. (2013) Front. Immunol. 3:408.
  2. Shibuya, A. et al. (1996) Immunity 4:573.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  11. Morimoto, K. et al. (2007) Oncogene 27:264.
  12. Chan, C.J. et al. (2014) Nat. Immunol. 15:431.
  13. Shibuya, K. et al. (1999) Immunity 11:615.
  14. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  15. Shibuya, A. et al. (1998) J. Immunol. 166:1671.

Long Name

DNAX Accessory Molecule 1

Alternate Names

CD226, DNAM1, PTA1, TLiSA1

Entrez Gene IDs

10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)

Gene Symbol

CD226

UniProt

Q15762.2

Additional DNAM-1/CD226 Products

Product Documents for Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF

For research use only

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