Recombinant Human EBI3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11084-EI

R&D Systems, part of Bio-Techne
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11084-EI-050
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Key Product Details

Source

CHO

Accession #

Q14213.2

Conjugate

Unconjugated

Applications

Bioactivity

View all EBI3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human EBI3 protein
Arg21-Lys220

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg21

Predicted Molecular Mass

23 kDa

SDS-PAGE

30-40 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (Catalog # 7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.

Scientific Data Images for Recombinant Human EBI3 Protein, CF

Recombinant Human EBI3 Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.

Recombinant Human EBI3 Protein SDS-PAGE.

2 μg/lane of Recombinant Human EBI3 Protein (Catalog # 11084-EI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-40 kDa.

Formulation, Preparation and Storage

11084-EI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBI3

EBI3 (Epstein-Barr virus-induced gene 3 protein) also known as IL-27B, is a secreted cytokine (1). It shares similar domain structure as IL-12B except there are only two fibronectin type -III domains instead of three. Through non-covalent interaction, EBI-3 binds to IL-12A (IL-12 p35), IL-23A (IL-23p19), or IL-27A (IL-27p28/IL-30) to form IL-35, IL-39, or IL-27, respectively (2-4). In addition to forming the heterodimeric cytokines, EBI-3 can also function on its own. Expression of EBI-3 is induced in neutrophils stimulated with TLR8 ligands, with no detectable increase in IL-35, IL-39 or IL-27 (5). EBI-3 also mediate IL-6 trans-signaling through receptor gp130 in B9 mouse plasmacytoma cells (6).

References

  1. Devergne, O. et al. (1996) J. Virol. 70:1143.
  2. Devergne, O. et al. (1997) Protc. Natl. Acad. Sci. U.S.A. 94:12041.
  3. Pflanz, S. et al. (2002) Immunity 16:779.
  4. Wang, X. et al. (2016) Eur. J. Immuno. 46:1343.
  5. Cassatella, M.A. et al. (2020) J Leukoc. Biol. 108:1515.
  6. Chehboun, S. et al. (2017) J Biol. Chem. 292:6644.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Entrez Gene IDs

10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey)

Gene Symbol

EBI3

UniProt

Q14213.2

Additional EBI3 Products

Product Documents for Recombinant Human EBI3 Protein, CF

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Product Specific Notices for Recombinant Human EBI3 Protein, CF

For research use only

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