Recombinant Human ECE-2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1645-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ECE-2 protein
Gly104-Trp787, with an N-terminal 10-His tag and Val
Gly104-Trp787, with an N-terminal 10-His tag and Val
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
79 kDa
SDS-PAGE
113 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is > 250 pmol/min/µg, as measured under the described conditions.
The specific activity is > 250 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1645-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ECE-2
ECE-2 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-1, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-2 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Three alternatively spliced forms differ in their cytoplasmic tail (2). In addition to big endothelin-1, ECE-2 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, substance P, dynorphin B, proenkephalin-derived peptides such as peptide E, BAM 22 and BAM18, and PEN-LEN, an endogenous inhibitor of prohormone convertase 1 (3). Together with ECE-1, it is also involved in degradation of beta-amyloid peptide (4). The ectodomain of human ECE-2 was expressed with an N-terminal His tag and purified.
References
- Turner, A.J. et al. (2001) BioEssays 23:261.
- Lorenzo, M.N. et al. (2001) Biochim. Biophys. Acta 1522:46.
- Mzhavia, N. et al. (2003) J. Biol. Chem. 278:14704.
- Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.
Long Name
Endothelin-converting Enzyme-2
Alternate Names
ECE2
Entrez Gene IDs
9718 (Human)
Gene Symbol
ECE2
UniProt
Additional ECE-2 Products
Product Documents for Recombinant Human ECE-2 Protein, CF
Product Specific Notices for Recombinant Human ECE-2 Protein, CF
For research use only
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