Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8894-FC

Low-affinity V176F polymorphic variant
R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

P08637

Conjugate

Unconjugated

Applications

Bioactivity

View all Fc gamma RIIIA/CD16a Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein
Gly17-Gln208, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly17 & Met18

Predicted Molecular Mass

23 kDa

SDS-PAGE

43-58 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Fc gamma RIIIa/CD16a (V176F) was immobilize on a Ms x PolyHis coated plate, it binds biotinylated Human IgG. The concentration of biotinylated Human IgG that produces 50% of the optimal binding response is approximately 0.5-2.5 μg/mL.

Scientific Data Images for Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF

Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein Binding Activity

Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein Binding Activity

When Recombinant Human Fc gamma RIIIa/CD16a (V176F) was immobilize on a Ms x PolyHis coated plate, it binds biotinylated Human IgG with an ED50 of 0.5-2.5 μg/mL.

Formulation, Preparation and Storage

8894-FC
Formulation Lyophilized from a 0.2 μm filtered solution in sterile PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIIA/CD16a

Fc gamma RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes (1-3). In humans, it is expressed as a 50-70 kDa transmembrane activating receptor on NK cells, T cells, monocytes, and macrophages (2). It is closely related to the GPI-linked Fc gamma RIIIB which is expressed on human neutrophils and eosinophils (1, 3). These two proteins share 97% amino acid (aa) identity within their extracellular domains (ECD) (4). The ECD of Fc gamma RIIIA also shares 63%, 61%, 65%, 59%, and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIA, feline CD16, bovine CD16, and porcine Fc gamma RIIIB, respectively. Mature human Fc gamma RIIIA consists of a 192 aa ECD with two C2-type Ig-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (5, 6). Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma-chain or CD3 zeta (7, 8). Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA (9). Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity (10-13). Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis (13). Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases (11, 12).

References

  1. Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
  2. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
  3. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
  4. Scallon, B.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5079.
  5. Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
  6. Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664.
  7. Kim, M.-K. et al. (2003) Blood 101:4479.
  8. Lanier, L.L. et al. (1989) Nature 342:803.
  9. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.    
  10. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
  11. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
  12. Masuda, M. et al. (2006) Atherosclerosis 188:377.
  13. Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.

Long Name

Fc gamma Receptor III A

Alternate Names

CD16a, FCGR3A, FcgRIIIA

Entrez Gene IDs

2214 (Human); 304966 (Rat)

Gene Symbol

FCGR3A

UniProt

P08637

Additional Fc gamma RIIIA/CD16a Products

Product Documents for Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF

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Product Specific Notices for Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF

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