Recombinant Human IL-12 Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT10018

Biotinylated, Linked Heterodimer, GS Linker, Defined heterodimer stoichiometry.
R&D Systems, part of Bio-Techne
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BT10018-025
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Key Product Details

Source

HEK293

Accession #

P29460.1, P29459.2

Structure / Form

GS-linked heterodimer
Biotinylated via Amines

Conjugate

Biotin

Applications

Bioactivity

View all IL-12 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-12 protein
Human IL-12p40
(Ile23-Ser328)
Accession # P29460.1		 GSGSSRGGSGSGGSGGGGSK Human IL-12p35
(Arg23-Ser219)
Accession # P29459.2
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile23

Predicted Molecular Mass

59 kDa

SDS-PAGE

68-80 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Whe Recombinant Human IL-12 R beta 1 Fc Chimera Protein  (Catalog # 839-B1) is immobilized at 1.00 µg/mL (100uL/well), Biotinylated Recombinant Human IL‑12 (Catalog # BT10018) binds with an ED50 of 40.0-400 ng/mL.

Scientific Data Images for Recombinant Human IL-12 Biotinylated Protein, CF

Recombinant Human IL-12 Biotinylated Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 R beta 1 Fc Chimera Protein (839-B1) is immobilized at 1.00 µg/mL (100µL/well), Biotinylated Recombinant Human IL‑12 Protein (Catalog # BT10018) binds with an ED50 of 40.0-400 ng/mL

Recombinant Human IL-12 Biotinylated Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-12 Biotinylated Protein (Catalog # BT10018) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-80 kDa.

Formulation, Preparation and Storage

BT10018
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines (1). The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF (2, 3). These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells (4). These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells (5). Current evidence indicates that IL‑12, produced by macrophages in response to infectious agents, is a central mediator of the cell‑mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

References

  1. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.
  2. Gearing, D. et al. (1991) Cell 66:9.
  3. Merberg, D. et al. (1992) Immunology Today 13:78.
  4. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074.
  5. Airoldi, I. et al. (2000) Journal of Immunology 165:6880.

Long Name

Interleukin 12

Alternate Names

CLMF1, IL12, IL12A, NKSF1

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Gene Symbol

IL12A

UniProt

P29460.1, P29459.2

Additional IL-12 Products

Product Documents for Recombinant Human IL-12 Biotinylated Protein, CF

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Product Specific Notices for Recombinant Human IL-12 Biotinylated Protein, CF

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