Recombinant Human IL-15R alpha His-tag Avi-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # AVI11068
Biotinylated
Key Product Details
Learn more about Avi-tag Biotinylated Proteins
Source
CHO
Accession #
Structure / Form
Biotinylated via Avi-tag
Conjugate
Biotin
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human IL-15R alpha protein
Human IL-15R alpha (Ile31-Thr205) Accession # Q13261.1 |
6-His tag | Avi-tag |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile31
Predicted Molecular Mass
21 kDa
SDS-PAGE
45-63 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-15 (Catalog # 247-ILB) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-15R alpha His-tag Avi-tag (Catalog # AVI11068) binds with an ED50 of 6.00-60.0 ng/mL.
When Recombinant Human IL-15 (Catalog # 247-ILB) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-15R alpha His-tag Avi-tag (Catalog # AVI11068) binds with an ED50 of 6.00-60.0 ng/mL.
Scientific Data Images for Recombinant Human IL-15R alpha His-tag Avi-tag Protein, CF
Biotinylated Recombinant Human IL-15R alpha His-tag Avi-tag Protein Binding Activity.
When Recombinant Human IL-15 (247-ILB) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-15R alpha His-tag Avi-tag Protein (Catalog # AVI11068) binds with an ED50 of 6.00-60.0 ng/mL.Biotinylated Recombinant Human IL-15R alpha His-tag Avi-tag Protein SDS-PAGE.
2 μg/lane of Biotinylated Recombinant Human IL-15R alpha His-tag Avi-tag Protein (Catalog # AVI11068) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-63 kDa.Formulation, Preparation and Storage
AVI11068
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-15R alpha
References
- Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
- Di Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
- Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
- Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
- Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
- Giri, J.G. et al. (1995) EMBO 14:3654.
- Grabstein, K. et al. (1994) Science 264:965.
- Giri, J. et al. (1994) EMBO J. 13:2822.
- Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
- Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
- Dubois, S. et al. (2002) Immunity 17:537.
- Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
- Neely, G.G. et al. (2004) J. Immunol. 172:4225.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
- Mortier, E. et al. (2004) J. Immunol. 173:1681.
Long Name
Interleukin 15 Receptor alpha
Alternate Names
CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA
Gene Symbol
IL15RA
UniProt
Additional IL-15R alpha Products
Product Documents for Recombinant Human IL-15R alpha His-tag Avi-tag Protein, CF
Product Specific Notices for Recombinant Human IL-15R alpha His-tag Avi-tag Protein, CF
For research use only
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