Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF

R&D Systems, part of Bio-Techne | Catalog # 5837-IL

R&D Systems, part of Bio-Techne
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5837-IL-010
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Key Product Details

Source

CHO

Accession #

Q16552 (IL-17A) & Q96PD4 (IL-17F)

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-17A/F Heterodimer Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-17A/F Heterodimer protein
Human IL-17A
(Gly24-Ala155)
Accession # Q16552
Human IL-17F
(Arg31-Gln163)
Accession # Q96PD4
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24 (IL-17A) & Arg21 (IL-17F)

Predicted Molecular Mass

15.1 kDa (IL-17A) & 14.9 kDa (IL-17F)

SDS-PAGE

20 & 16 kDa, reducing conditions

Activity

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.4-2 ng/mL.

Reviewed Applications

Read 2 reviews rated 5 using 5837-IL in the following applications:

Formulation, Preparation and Storage

5837-IL
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCI.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17A/F Heterodimer

Human IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (3-5). Mature human IL-17A and IL-17F share 61% and 56% amino acid sequence identity with mouse IL-17A and IL-17F, respectively. They share 50% aa sequence identity with each other. IL-17A/F and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

References

  1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  2. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  4. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  5. Gu, C. et al. (2013) Cytokine 64:477.
  6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
  7. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  8. Ouyang, W. et al. (2008) Immunity 28:454.
  9. Steinman, L. (2007) Nat. Med. 13:139.
  10. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  11. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  13. Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.

Long Name

Interleukin 17 A/F

Alternate Names

CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, Cytotoxic T-Lymphocyte-Associated Protein 8, IL17, IL-17, IL-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A, interleukin-17A

UniProt

Q16552 (IL-17A) & Q96PD4 (IL-17F)

Additional IL-17A/F Heterodimer Products

Product Documents for Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF

For research use only

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