Recombinant Human IL-2 Heat Stable Agonist Protein, CF

Predictive analytics are increasingly effective in solving challenges associated with developing protein reagents for cell culture applications, including thermal and pH instability. We have improved the stability of IL-2 by generating a synthetic structural core while maintaining efficiency in engagement and activation of IL-2 R beta/ gamma_c receptors. The enhanced IL-2 displays improved heat stability enabling it to retain bioactivity while withstanding elevated temperatures and extended culture durations. Recombinant IL-2 is a bioactive protein for use in cell culture applications, often used for the expansion of T cells. IL-2 is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils (1-3).  rhIL-2 is also used for the expansion of NK cells, early differentiated T cells and effector memory Treg cells for adoptive cell transfer cancer immunotherapy (4,5).  rhIL-2 Heat Stable agonist is verified for activity using an NK92 proliferation assay as well as T Cell culture applications comparing to the commonly used mutant aldesleukin IL-2 version.