Recombinant Human LILRB1/CD85j/ILT2 His Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI8989

Biotinylated
R&D Systems, part of Bio-Techne
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AVI8989-050
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Key Product Details

Source

CHO

Accession #

Q8NHL6.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all LILRB1/CD85j/ILT2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LILRB1/CD85j/ILT2 protein
Human LILRB1/CD85j/ILT2
(Gly24-Arg457)
Accession # Q8NHL6.1		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

50 kDa

SDS-PAGE

70-80 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human LILRB1/CD85j/ILT2 Antibody (Catalog # MAB20172) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human LILRB1/CD85j/ILT2 His-tag Avi-tag (Catalog # AVI8989) binds with an ED50 of 0.600-9.00 ng/mL.

Scientific Data Images for Recombinant Human LILRB1/CD85j/ILT2 His Avi-tag Protein, CF

Biotinylated Recombinant Human LILRB1/CD85j/ILT2 His-tag Avi-tag Protein Binding Activity.

When Human LILRB1/CD85j/ILT2 Antibody (MAB20172) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human LILRB1/CD85j/ILT2 His-tag Avi-tag Protein (Catalog # AVI8989) binds with an ED50 of 0.600-9.00 ng/mL.

Biotinylated Recombinant Human LILRB1/CD85j/ILT2 His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human LILRB1/CD85j/ILT2 His-tag Avi-tag Protein (Catalog # AVI8989) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-80 kDa.

Formulation, Preparation and Storage

AVI8989
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB1/CD85j/ILT2

LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family (1). Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs (2). Alternative splicing generates an additional isoform that lacks the transmembrane and cytoplasmic regions (3). LILRB1 is expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma delta T cells, and monocyte-derived dendritic cells (3-7). LILRB1 binds to MHC-I as well as non-classical MHC-I molecules (e.g. HLA-F, HLA-G, and HLA-B27) and the MHC-I mimetic UL18 encoded by cytomegalovirus (3, 5, 8-10). R&D Systems in-house testing indicates that LILRB1 also binds to Angiopoietin-like 7. Ligation of LILRB1 inhibits the antigen induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma delta T cells (3, 4, 11-13). On dendritic cells, ligation inhibits the production of IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis (7). Our Avi-tag Biotinylated human LILRB1 His-tag protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Samaridis, J. and M. Colonna (1997) Eur. J. Immunol. 27:660.
  3. Colonna, M. et al. (1997) J. Exp. Med. 186:1809.
  4. Harly, C. et al. (2011) Blood 117:2864.
  5. Cosman, D. et al. (1997) Immunity 7:273.
  6. Young, N.T. et al. (2001) J. Immunol. 166:3933.
  7. Young, N.T. et al. (2008) Blood 111:3090.
  8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
  9. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
  10. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  11. Ince, M.N. et al. (2004) Immunology 112:531.
  12. Saverino, D. et al. (2000) J. Immunol. 165:3742.
  13. Saverino, D. et al. (2002) J. Immunol. 168:207.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1

Alternate Names

CD85j, ILT2, LIR1, MIR7

Entrez Gene IDs

10859 (Human)

Gene Symbol

LILRB1

UniProt

Q8NHL6.1

Additional LILRB1/CD85j/ILT2 Products

Product Documents for Recombinant Human LILRB1/CD85j/ILT2 His Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human LILRB1/CD85j/ILT2 His Avi-tag Protein, CF

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