Recombinant Human LILRB1/CD85j/ILT2 His Biotin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT8989

Biotinylated
R&D Systems, part of Bio-Techne
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BT8989-050
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Key Product Details

Source

HEK293

Accession #

Q8NHL6.1

Structure / Form

Biotinylated via amines

Conjugate

Biotin

Applications

Bioactivity

View all LILRB1/CD85j/ILT2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LILRB1/CD85j/ILT2 protein
Gly24-His458, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

48 kDa

SDS-PAGE

68-79 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human LILRB1/CD85j/ILT2 Antibody (Catalog # MAB20172) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human LILRB1/CD85j/ILT2 His-tag (Catalog # BT8989) binds with an ED50 of 1.00-15.0 ng/mL.

Scientific Data Images for Recombinant Human LILRB1/CD85j/ILT2 His Biotin Protein, CF

Biotinylated Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein Binding Activity.

When Human LILRB1/CD85j/ILT2 Antibody (MAB20172) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein (Catalog # BT8989) binds with an ED50 of 1.00-15.0 ng/mL.

Biotinylated Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human LILRB1/CD85j/ILT2 His-tag Biotinylated Protein (Catalog # BT8989) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-79 kDa.

Formulation, Preparation and Storage

BT8989
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB1/CD85j/ILT2

LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family (1). Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs (2). Alternative splicing generates an additional isoform that lacks the transmembrane and cytoplasmic regions (3). LILRB1 is expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma delta T cells, and monocyte-derived dendritic cells (3-7). LILRB1 binds to MHC-I as well as non-classical MHC-I molecules (e.g. HLA-F, HLA-G, and HLA-B27) and the MHC-I mimetic UL18 encoded by cytomegalovirus (3, 5, 8-10). R&D Systems in-house testing indicates that LILRB1 also binds to Angiopoietin-like 7. Ligation of LILRB1 inhibits the antigen induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma delta T cells (3, 4, 11-13). On dendritic cells, ligation inhibits the production of IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis (7).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Samaridis, J. and M. Colonna (1997) Eur. J. Immunol. 27:660.
  3. Colonna, M. et al. (1997) J. Exp. Med. 186:1809.
  4. Harly, C. et al. (2011) Blood 117:2864.
  5. Cosman, D. et al. (1997) Immunity 7:273.
  6. Young, N.T. et al. (2001) J. Immunol. 166:3933.
  7. Young, N.T. et al. (2008) Blood 111:3090.
  8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
  9. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
  10. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  11. Ince, M.N. et al. (2004) Immunology 112:531.
  12. Saverino, D. et al. (2000) J. Immunol. 165:3742.
  13. Saverino, D. et al. (2002) J. Immunol. 168:207.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1

Alternate Names

CD85j, ILT2, LIR1, MIR7

Entrez Gene IDs

10859 (Human)

Gene Symbol

LILRB1

UniProt

Q8NHL6.1

Additional LILRB1/CD85j/ILT2 Products

Product Documents for Recombinant Human LILRB1/CD85j/ILT2 His Biotin Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human LILRB1/CD85j/ILT2 His Biotin Protein, CF

For research use only

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