Recombinant Human Ly-6G6F Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10513-L6

R&D Systems, part of Bio-Techne
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10513-L6-050
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Key Product Details

Source

HEK293

Accession #

Q5SQ64.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Ly-6G6F Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Ly-6G6F protein
Human Ly-6G6F
(Ala17-Trp235)
Accession # Q5SQ64.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala17

Predicted Molecular Mass

50 kDa

SDS-PAGE

55-62 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect 1-10 μg/mL.

Scientific Data Images for Recombinant Human Ly-6G6F Fc Chimera Protein, CF

Recombinant Human Ly-6G6F Fc Chimera Protein Bioactivity

Recombinant Human Ly-6G6F Fc Chimera Protein Bioactivity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 1-10 μg/mL.
Recombinant Human Ly-6G6F Fc Chimera Protein SDS-PAGE

Recombinant Human Ly-6G6F Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human Ly-6G6F Fc Chimera Protein (10513-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-62 kDa and 110-124 kDa, respectively.

Formulation, Preparation and Storage

10513-L6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly-6G6F

Lymphocyte antigen 6 complex locus protein G6f, also known as G6f, is a 32-kDa single pass type I membrane protein belonging to the Immunoglobulin Superfamily that is encoded in the MHC (1). The extracellular domain is 281 aa in length and contains one disulfide bond (C35 ↔ C106) and an N-linked glycosylation site at S88. The ECD of human Ly-6G6F shares 64.5% and 63% sequence identity with mouse and rat Ly-6G6F, respectively. The intracellular tail of Ly-6G6F is 40 amino acids in length and contains one tyrosine residue (Y281), which is phosphorylated after treatment of cells with pervanadate. Ly-6G6F may play a role in signal transduction pathways involved with Grb2 and Grb7, such as the Ras-MAP kinase pathway (1). Ly-6G6F tyrosine phosphorylation was also found to take place in response to collagen, although not in response to the G protein-coupled receptor agonists, thrombin and ADP. A global proteomics approach identifies Ly-6G6F as a novel phosphorylated signaling proteins in GPVI-activated platelets (2, 3).  Leukocytes mRNA levels of Ly-6G6F mainly involved in inflammation were significantly higher in patients carrying granulin mutations compared with asymptomatic carriers (4).

References

  1. De Vet, E.C. et al. (2003) Biochem. J. 375:207.
  2. Garcia, A. et al. (2006) Proteomics. 6:5332.
  3. Lewandrowski U. et al. (2006) Mol. Cell Proteomics 5:226.
  4. Milanesi E. et al. (2013) Neurobiol. Aging. 34:1837.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

C6orf21, G6f, LY6G6D, LY6G6F, NG32

Entrez Gene IDs

259215 (Human); 433099 (Mouse); 309609 (Rat)

Gene Symbol

LY6G6F

UniProt

Q5SQ64.2

Additional Ly-6G6F Products

Product Documents for Recombinant Human Ly-6G6F Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Ly-6G6F Fc Chimera Protein, CF

For research use only

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