Recombinant Human Mer His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10515

Biotinylated
R&D Systems, part of Bio-Techne
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AVI10515-050
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Key Product Details

Source

HEK293

Accession #

Q12866.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Mer Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Mer protein
Human Mer
(Arg20-Asp500)
Accession # Q12866.2		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg20

Predicted Molecular Mass

55 kDa

SDS-PAGE

108-121 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human Mer His-tag Avi-tag (Catalog# AVI10515) is captured on EvenCoat Streptavidin Coated Plates  (Catalog # CP004) at 2.00 µg/mL (100 µL/well). Recombinant Human Gas6 Protein (Catalog # 885-GSB) binds with an ED50 of 80.0‑800 ng/mL.

Scientific Data Images for Recombinant Human Mer His-tag Avi-tag Protein, CF

Recombinant Human Mer His-tag Avi-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human Mer His-tag Avi-tag Protein (Catalog # AVI10515) is captured on EvenCoat Streptavidin Coated Plates (CP004) at 2.00 µg/mL (100 µL/well), Recombinant Human Gas6 Protein (885-GSB) binds with an ED50 of 80.0-800 ng/mL.

Recombinant Human Mer His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Mer His-tag Avi-tag Protein (Catalog # AVI10515) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 108-121 kDa.

Formulation, Preparation and Storage

AVI10515
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Tyrosine-protein Kinase Mer, also known as c-Mer and MerTK, is a member of the receptor tyrosine kinase subfamily TAM (Tyro3, Axl, and Mer). Mature human Mer consists of 485 aa extracellular domain, 21 aa transmembrane domain, and 473 aa cytoplasmic domain. Within the extracellular domain, human Mer shares 77.2% and 76.6% homology with mouse and rat Mer, respectively. Similar to Axl and Tyro3, the extracelluar domain of Mer contains two Ig-like motifs and two fibronectin type III motifs. Mer is not expressed in normal B- and T-cells but expressed in neoplastic B- and T-cell lines (1-2).  It is also show higher expression in immunosuppressive M2‑like macrophages (3).  Mer is known to bind Gas6, Protein S, Tubby, Tubby-like protein 1 (Tulp1), and Galectin-3 (4-7). Upon binding ligands via the Ig-like motif, Mer is dimerized to trans-autophosphorylate the kinase domain to induce downstream signaling. It has been shown that Mer signaling in macrophages induces M2 polarization, which promote tumor growth, metastasis and evasion of anti-tumor immunity in tumor microenviroment (8). Inhibition of Mer, especially on leukocytes and macrophages, is an effective anti-cancer therapy (9). Our Avi-tag Biotinylated Mer His-tag features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Graham, D.K. et al. (1994) Cell Growth Differ. 5:647.
  2. Graham, D.K. et al. (2006) Clin. Cancer Res. 12:2662.
  3. Shibata, T. et al. (2014) J. Immunol. 192:3569.
  4. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  5. Uehara, H. et al. (2008) J. Immunol. 180:2522.
  6. Caberoy, N.B. et al. (2010) EMBO J. 29:3898.
  7. Caberoy, N.B. et al. (2012) J. Cell Physiol. 227:401.
  8. Kim, S.Y. et al. (2016) Sci. Rep. 6:29673.
  9. Cummings, C.T. et al. (2013) Clin. Cancer Res. 19:5275.

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Gene Symbol

MERTK

UniProt

Q12866.2

Additional Mer Products

Product Documents for Recombinant Human Mer His-tag Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human Mer His-tag Avi-tag Protein, CF

For research use only

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