Recombinant Human/Mouse/Rat GDF-11/BMP-11 Protein Best Seller

R&D Systems, part of Bio-Techne | Catalog # 1958-GD

R&D Systems, part of Bio-Techne
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Carrier Free
1958-GD-010/CF
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1958-GD-010
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Key Product Details

Source

E. coli

Accession #

O95390

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all GDF-11/BMP-11 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived GDF-11/BMP-11 protein
Asn299-Ser407, with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met-Asn299-Leu-Gly-Leu-Asp-(Cys)-Asp-Glu-His

Predicted Molecular Mass

12.6 kDa (monomer)

Activity

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.
The ED50 for this effect is 0.8-4.8 ng/mL.

Scientific Data Images for Recombinant Human/Mouse/Rat GDF-11/BMP-11 Protein

Graph showing bioactivity of GDF-11 protein

Bioactivity of Recombinant GDF-11 Protein

Recombinant Human/Mouse/Rat GDF‑11 protein 1958-GD induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 0.8-4.8 ng/mL.
Graph showing bioactivity of GDF-11 protein

Bioactivity of Recombinant GDF-11 Protein

1 μg/lane of Recombinant Human/Mouse/Rat GDF‑11 1958-GD protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 13 kDa and 21 kDa, respectively.
Graph showing bioactivity of GDF-11 protein

Bioactivity of Recombinant GDF-11 Protein

ESI analysis of Recombinant Human/Mouse/Rat GDF-11 protein 1958-GD. The peak at 25163 Da corresponds to the calculated molecular mass of the disulfide-linked homodimer.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1958-GD
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1958-GD/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-11/BMP-11

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide which contains a signal sequence for secretion and an RXXR proteolytic processing site to yield a 109 aa residue carboxy-terminal mature protein (1). Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99-100% identical. GDF-11 and GDF-8 share 90% amino acid sequence identity within the mature protein. As detected by in situ hybridization, GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1, 2). Targeted deletion of GDF-11, in mice, results in a spectrum of abnormalities including palatal malformation, vertebral defects, elongated trunks with a reduced or absent tail, missing or malformed kidneys, and an increased number of neurons in the olfactory epithelium (2-5). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (6). Systemic GDF-11 levels decline with age and administration of higher levels of GDF-11 can reverse age-related cardiac hypertrophy (7). In addition, systemic administration of recombinant GDF-11 protein restores genomic integrity and health of muscle stem cells, neurovasculature and enhances neurogenesis (8, 9). R&D Systems recombinant GDF-11 preparations have been shown to act similarly to GDF-8 in both the Xenopus animal cap and the K562 assays.

References

  1. Gamer, L.W. et al. (1999) Dev. Biol. 208: 222. 
  2. Nakashima, M. et al. (1999) Mech. Dev. 80:185.  
  3. Gad, J.M. and P.P.L. Tam (1999) Curr. Biol. 9:R783.  
  4. McPherron, A.C. et al. (1999) Nat. Genet. 22:260.
  5. Esquela, A.F. and S.J. Lee (2003) Dev. Biol. 257:356.
  6. Oh, S.P. et al. (2002) Genes & Dev. 16:274.
  7. Loffredo, F.S. et. al. (2013) Cell. 153.828.
  8. Katsimpardi, L. et. al. (2014) Science (ahead of print).
  9. Sinha, M. et. al. (2014) Science (ahead of print).

Long Name

Growth Differentiation Factor 11

Alternate Names

BMP-11, BMP11, GDF11

Entrez Gene IDs

10220 (Human)

Gene Symbol

GDF11

UniProt

O95390

Additional GDF-11/BMP-11 Products

Product Documents for Recombinant Human/Mouse/Rat GDF-11/BMP-11 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 1958-GD (with carrier)
Download SDS 1958-GD/CF (carrier free)

Product Specific Notices for Recombinant Human/Mouse/Rat GDF-11/BMP-11 Protein

For research use only

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