Recombinant Human Neurofascin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8208-NF

R&D Systems, part of Bio-Techne
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8208-NF-050
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Key Product Details

Source

HEK293

Accession #

NP_055905.2

Conjugate

Unconjugated

Applications

Bioactivity

View all Neurofascin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Neurofascin protein
Ile25-Trp1039, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile25

Predicted Molecular Mass

116 kDa

SDS-PAGE

120-140 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Neurofascin, immobilized at 6 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Formulation, Preparation and Storage

8208-NF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurofascin

Neurofascin, a type I transmembrane glycoprotein, is a member of the L1 family of cell adhesion molecules (CAMs) (1-4). L1CAM family members are composed of an extracellular domain (ECD) that contains six immunoglobulin (Ig)-like domains and multiple fibronectin type III repeats, followed by transmembrane and cytoplasmic domains (2, 4, 5). Multiple isoforms of Neurofascin, including NF155, NF166, NF180, and NF186, can be generated by alternative splicing with a predicted range of approximately 70 kDa to 150 kDa (4). These isoforms differ in the combination of fibronectin type III repeats, as well as in the presence of a proline-, alanine-, and threonine-rich segment (PAT domain) located just after the fourth fibronectin type III repeat (4). This recombinant human Neurofascin protein is most close to NF155 and shares 96% amino acid sequence identity with comparable regions of rat and mouse homologs. In rats, NF155 is transiently expressed by oligodendrocytes and Schwann cells during axon myelination (6, 7). NF155 clusters in paranodal regions of oligodendroglia and binds to the Caspr-Contactin complex located on the adjacent axon to form and stabilize paranodal axoglial junctions (8-11). It has been suggested that the ECD of NF155 must be cleaved from oligodendroglia membranes to form and/or stabilize the paranodal structure (12). NF155 has also been shown to promote neuronal adhesion and neurite outgrowth in rats and chickens (12-14). Alterations in NF155 expression have been associated with multiple sclerosis (15).

References

  1. Rathjen, F.G. et al. (1987) Cell 51:841.
  2. Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
  3. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
  4. Kriebel, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:694.
  5. Liu, H. et al. (2011) J. Biol. Chem. 286:797.
  6. Collinson, J.M. et al. (1998) Glia 23:11.
  7. Basak, S. et al. (2007) Dev. Biol. 311:408.
  8. Charles, P. et al. (2002) Curr. Biol. 12:217.
  9. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
  10. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
  11. Sherman, D.L. et al. (2005) Neuron 48:737.
  12. Maier, O. et al. (2006) Exp. Cell Res. 312:500.
  13. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  14. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
  15. Howell, O.W. et al. (2006) Brain 129:3173.

Alternate Names

NFASC, NRCAML

Entrez Gene IDs

23114 (Human); 269116 (Mouse); 116690 (Rat)

Gene Symbol

NFASC

UniProt

NP_055905.2

Additional Neurofascin Products

Product Documents for Recombinant Human Neurofascin Protein, CF

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Product Specific Notices for Recombinant Human Neurofascin Protein, CF

For research use only

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