Recombinant Human PILR-alpha Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10702

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q9UKJ1.3

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all PILR-alpha Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human PILR-alpha protein
Human PILR-alpha
(Gln20-Thr196)
Accession # Q9UKJ1.3		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr25

Predicted Molecular Mass

48 kDa

SDS-PAGE

60-70 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its binding ability in a functional ELISA.
When Recombinant Human PANP Fc Chimera (Catalog # 7920-PN) is coated at 5 μg/mL, 100 μL/well, Biotinylated Recombinant Human PILR‑ alpha Fc Chimera Avi-tag (Catalog # AVI10702) binds with an ED50 of 0.6-6 μg/mL.

Scientific Data Images for Recombinant Human PILR-alpha Fc Chimera Avi-tag Protein, CF

Recombinant Human PILR-alpha Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human PANP Fc Chimera (7920-PN) is coated at 5 μg/mL, 100 uL/well, Biotinylated Recombinant Human PILR‑ alpha Fc Chimera Avi-tag (Catalog # AVI10702) binds with an ED50 of 0.6-6 μg/mL.

Formulation, Preparation and Storage

AVI10702
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: PILR-alpha

Paired immunoglobulin-like type 2 receptor-alpha (PILRA) is one of two members that belong to a small family of immunoregulatory Ig-superfamily receptors (1-4). It is a counterpart to PILRB and the PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation (1,2). Mature human PILRA consists of an extracellular domain (ECD) with one V-type Ig-like domain, a transmembrane domain, and a cytoplasmic domain with two immunoreceptor Tyr‑based inhibitory (ITIM) motifs. Given that ITIMs are known to interact with phosphatases such as PTPN6 and PTPN11, the presence of these motifs makes PILRA an inhibitory receptor (1-4). The ECD of human PILRA shares 42% and 40% amino acid sequence identity with mouse and rat PILRA, respectively. Three potential isoforms for human PILRA have been reported. PILRA is expressed by neutrophils, macrophages, monocytes, mast cells, APCs, microglia, neurons, cardiac muscle and renal proximal plus pancreatic duct eipthelium (4, 7, 8). It has multiple binding partners, including CD99 (4, 9), glycoprotein B/gB of HSV1 (in human) (7), PANP (PILR-associated neural protein) (8) and NPDC1 plus collectin-12 (10). Although PILRA and PILRB are highly similar in their ECD amino acid sequence, they do not necessarily share the same ligands, as PILRB fails to bind to gB and PANP (8, 10). PILRA binding appears to be dependent upon the presence of a poorly-defined peptide sequence coupled to a sialylated, O-linked carbohydrate motif but its exact function remains unclear (5, 9-12). Up regulation of PILRA in the early stage of immune reaction and its subsequent binding to CD99 may lead to a downregulation of the inflammatory response (10). Genome-wide association studies (GWAS) have linked PILRA to Alzheimer's Disease (AD) through association of PILRB with ZCWPW1 (13,14). It was further supported that ZCWPW1 locus SNP rs1476679 was associated with reduced PILRA levels suggesting a potential role for the gene in AD (15). The missense variant (G78R, rs1859788) of PILRA is thought to be the causal allele for the confirmed AD risk locus. The variant reduced the binding of PILRA to several ligands including a novel ligand, complement component 4A, and herpes simplex virus 1 (HSV-1) glycoprotein B (16). The observed protection from AD risk by PILRA G78R variant provided a new candidate for therapeutic target. Our Avi-tag Biotinylated human PILRA Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
  2. Lanier, L.L. (2001) Curr. Opin. Immunol. 13:326.
  3. Fournier, N. et al. (2000) J. Immunol. 165:1197.
  4. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
  5. Mousseau, D.D. et al. (2000) J. Biol. Chem. 275:4467.
  6. SwissProt Accession # Q9UKJ1.
  7. Tato, C.M. et al. (2012) PLoS ONE 7:e31680.
  8. Satoh, T. et al. (2008) Cell 132:935.
  9. Tabata, S. et al. (2008),J. Biol. Chem. 283:8893.
  10. Sun, Y. et al. (2012) J. Biol. Chem. 287:15837.
  11. Wang, J. et al. (2008) J. Biol. Chem. 180:1686.
  12. Arii, J. et al. (2010) J. Virol. 84:10733.
  13. Karch, CM. et al. (2016) PLoS ONE. 11:1-22.
  14. Allen, M. et al. (2015) Neurol Genet. 1:e15 (PubMed: 27066552).
  15. Patel, T. et al. (2018) Neurolpathol Appl Neurobiol. 44(5):506-521.
  16. Rathore, N. et al. (2018) PLoS Genet. 14(11):e1007427.

Long Name

Paired-Ig-likeType 2 Receptor alpha

Alternate Names

FDF03, PILRA, PILRalpha

Entrez Gene IDs

29992 (Human); 231805 (Mouse); 288568 (Rat)

Gene Symbol

PILRA

UniProt

Q9UKJ1.3

Additional PILR-alpha Products

Product Documents for Recombinant Human PILR-alpha Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human PILR-alpha Fc Chimera Avi-tag Protein, CF

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