Recombinant Human TACE/ADAM17 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 930-ADB
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Mature form. Recombinant Human TACE/ADAM17 may be prone to proteolytic cleavage at C-terminus. The poly-His tag may not be present in the preparation.
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human TACE/ADAM17 protein
Arg215-Asn671, with a C-terminal 6-His tag
Arg215-Asn671, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg215
Predicted Molecular Mass
52 kDa
SDS-PAGE
64 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003).
The specific activity is >500 pmol/min/µg, as measured under the described conditions.
The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 4 using 930-ADB in the following applications:
Formulation, Preparation and Storage
930-ADB
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Brij-35. |
| Reconstitution |
Reconstitute at 0.2 mg/mL in sterile, deionized water.
|
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TACE/ADAM17
TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of tumor necrosis factor-alpha (TNF-alpha) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.
References
- Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha-Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
- Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.
Long Name
TNF-alpha Converting Enzyme
Alternate Names
ADAM17, CD156b
Gene Symbol
ADAM17
UniProt
Additional TACE/ADAM17 Products
Product Documents for Recombinant Human TACE/ADAM17 Protein, CF
Product Specific Notices for Recombinant Human TACE/ADAM17 Protein, CF
For research use only
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