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Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.050-0.500 ng/mL.
The specific activity of Recombinant Human Thrombopoietin is >1 x 107 units/mg, which is calibrated against the human Thrombopoietin reference standard (NIBSC code: 03/124).
Scientific Data Examples for Recombinant Human Thrombopoietin/TPO Protein, Animal-Free
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Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human Thrombopoietin/Tpo proteins.
Equivalent bioactivity of GMP (BT-TPO-GMP), Animal-Free (Catalog # BT-TPO-AFL) and RUO (BT-TPO) grades of Recombinant Human Thrombopoietin/Tpo proteins as measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells (orange, green, red, respectively).
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Animal-Free™ Recombinant Human Thrombopoietin/Tpo Protein Bioactivity.
Animal-Free™ Recombinant Human Thrombopoietin/Tpo (Catalog # BT-TPO-AFL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.050‑0.500 ng/mL.
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Animal-Free™ Recombinant Human Thrombopoietin/Tpo Protein SDS-PAGE.
2 μg/lane of Animal-Free™ Recombinant Human Thrombopoietin/Tpo Protein (Catalog # BT-TPO-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18‑19 kDa, under reducing conditions.
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Manufacturing Specifications
Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.
Production
- All molecular biology procedures use animal-free media and dedicated labware.
- Dedicated fermentors are utilized in committed animal-free areas.
Purification
- Protein purification columns are animal-free.
- Bulk proteins are filtered using animal-free filters.
- Purified proteins are stored in animal-free containers in a dedicated cold storage room.
Quality Assurance
- Low Endotoxin Level.
- No impairment of biological activity.
- High quality product obtained under stringent conditions.
- For ex vivo research or bioproduction, additional documentation can be provided.
Please read our complete Animal-Free Statement
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Product Documents for Recombinant Human Thrombopoietin/TPO Protein, Animal-Free
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