Recombinant Human Thrombopoietin/TPO Protein, CF

Name: Recombinant Human Thrombopoietin/TPO Protein, CF
Brand: R&D Systems
SKU: BT-TPO

R&D Systems, part of Bio-Techne | Catalog # BT-TPO

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Product Datasheet / COA / SDS

Catalog #

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Key Product Details

Source:	E. coli
Accession #:	NP_000451.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Thrombopoietin/TPO Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human Thrombopoietin/TPO Protein, CF

Source

E. coli-derived human Thrombopoietin/Tpo protein
Ser22-Leu195

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala-Ser22

Predicted Molecular Mass

19 kDa

SDS-PAGE

19 kDa

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED₅₀ for this effect is 0.0500-0.500 ng/mL.

Scientific Data Examples for Recombinant Human Thrombopoietin/TPO Protein, CF

	Equivalent Bioactivity of GMP,Animal-Free, and RUO gradesof Recombinant HumanThrombopoietin/Tpo proteins. Equivalent bioactivity of GMP(BT-TPO-GMP),Animal-Free (BT-TPO-AFL) and RUO (Catalog # BT-TPO) grades of RecombinantHuman Thrombopoietin/Tpoproteins as measured in a cellproliferation assay using MO7ehuman megakaryocytic leukemiccells (orange, green, red,respectively).
	Recombinant Human Thrombopoietin/Tpo Protein Bioactivity. Recombinant Human Thrombopoietin/Tpo (Catalog # BT-TPO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line. The ED₅₀ for this effect is 0.0500‑0.500 ng/mL.
	Recombinant Human Thrombopoietin/Tpo Protein SDS-PAGE. 2 μg/lane of Recombinant Human Thrombopoietin/Tpo Protein (Catalog # BT-TPO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-19 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-TPO

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution	Reconstitute the 10 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombopoietin/Tpo

Thrombopoietin (TPO) is a crucial regulator of hematopoietic stem cell (HSC) differentiation, maturation, and proliferation, as well as megakaryocytopoiesis and thrombopoiesis. TPO is often used in conjunction with Stem Cell Factor (SCF) and Flt-3 ligand in cell culture protocols to expand HSCs for bone marrow transplantation and cellular therapies. When combined with SCF and Flt-3 ligand, it promotes the differentiation of HSCs into megakaryocytes, leading to the production of platelets. It has shown promise in emerging cellular therapies for treating sickle cell disease, beta thalassemia, and other blood-related disorders. Moreover, TPO in combination with other cytokines is utilized in the generation of iNK cells, which have the potential to be incorporated into cellular immunotherapies.

Item	Value
Entrez Gene IDs	7066 (Human); 21832 (Mouse); 81811 (Rat)
Alternate Names	MGDF, MGDFC-mpl ligand, MK-CSF, MKCSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, TPOMKCSF, Thrombopoietin, Tpo, megakaryocyte stimulating factor, thrombopoietin nirs variant 1

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Recombinant Human Thrombopoietin/TPO Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT-TPO

Key Product Details

Product Specifications for Recombinant Human Thrombopoietin/TPO Protein, CF

Scientific Data Examples for Recombinant Human Thrombopoietin/TPO Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Thrombopoietin/Tpo

Customer Reviews for Recombinant Human Thrombopoietin/TPO Protein, CF

Product Documents for Recombinant Human Thrombopoietin/TPO Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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