Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 938-VC

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

P33151

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VE-Cadherin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human VE-Cadherin protein
Human VE-Cadherin
(Asp48-Gln593)
Accession # P33151		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp48

Predicted Molecular Mass

89 kDa (monomer)

SDS-PAGE

116 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.
The ED50 for this effect is 40-160 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

938-VC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

VE-Cadherin, also known as Cadherin-5 and CD144, is a member of the atypical/type II subgroup of Cadherin homophilic adhesion proteins (1). Mature human VE-Cadherin is a 125-135 kDa transmembrane glycoprotein that consistis of a 552 amino acid (aa) extracellular domain (ECD) with five Ca++-binding Cadherin domains, a 21 aa transmembrane segment, and a 164 aa cytoplasmic domain (2, 3). Within the ECD, human VE-Cadherin shares approximately 75% aa sequence identity with mouse and rat VE-Cadherin. A 90 kDa portion of the VE-Cadherin ECD can be shed in a metalloproteinase-dependent mechanism and may function as a VE-Cadherin antagonist (4, 5). VE-Cadherin is expressed on the surface of vascular endothelial cells (EC) from early in embryogenesis through adulthood as well as in the placenta, on hematopoietic cell progenitors, and on a subpopulation of hematopoietic stem cells (6-8). It is a major protein component of adherens junctions between cells of the endothelium (9). VE-Cadherin supports the extension and stabilization of existing vascular sprouts (10). VE-Cadherin also regulates or is regulated by VEGF R2, type I and type II TGF-beta receptors, and other endothelial junction proteins such as JAM-C, Claudin-5, and N-Cadherin (11-15). Elevated levels of soluble VE-Cadherin are found in the serum of myocardial infarction, angina pectoris, rheumatoid arthritis, and colorectal cancer patients (16-18).

References

  1. Giannotta, M. et al. (2013) Dev. Cell 26:441.
  2. Breviario, F. et al. (1995) Arterioscler. Throm. Vasc. Biol. 15:1229.
  3. Geyer, H. et al. (1999) Glycobiology 9:915.
  4. Herren, B. et al. (1998) Mol. Biol. Cell 9:1589.
  5. Li, H. et al. (2010) Cancer Gene Ther. 17:700.
  6. Breier, G. et al. (1996) Blood 87:630.
  7. Ema, M. et al. (2006) Blood 108:4018.
  8. Oberlin, E. et al. (2010) Blood 116:4444.
  9. Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.
  10. Perryn, E.D. et al. (2008) Dev. Biol. 313:545.
  11. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
  12. Orlova, V.V. et al. (2006) J. Exp. Med. 203:2703.
  13. Taddei, A. et al. (2008) Nat. Cell Biol. 10:923.
  14. Luo, Y. and G.L. Radice (2005) J. Cell Biol. 169:29.
  15. Rudini, N. et al. (2008) EMBO J. 27:993.
  16. Soeki, T. et al. (2004) Circ. J. 68:1.
  17. Sulkowska, M. et al. (2006) Tumori 92:67.
  18. Sidibe, A. et al. (2012) Arthritis Rheum. 64:77.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Entrez Gene IDs

1003 (Human); 12562 (Mouse)

Gene Symbol

CDH5

UniProt

P33151

Additional VE-Cadherin Products

Product Documents for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

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