Recombinant Human VSIG4 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI4646

Biotinylated
R&D Systems, part of Bio-Techne
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AVI4646-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Q9Y279.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all VSIG4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human VSIG4 protein
Human VSIG4
(Arg20-Pro283)
Accession # Q9Y279.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg20

Predicted Molecular Mass

58 kDa

SDS-PAGE

65-85 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its binding ability in a functional ELISA.
When Human iC3b is Immobilized at 2.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human VSIG4 Fc Chimera Avi-tag (AVI4646) that produces 50% optimal binding response is found to be approximately 5-40 ng/mL.

Scientific Data Images for Recombinant Human VSIG4 Fc Chimera Avi-tag Protein, CF

Recombinant Human VSIG4 Fc Chimera Avi-tag Protein Binding Activity

Recombinant Human VSIG4 Fc Chimera Avi-tag Protein Binding Activity

When Human iC3b is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human VSIG4 Fc Chimera Avi-tag (AVI4646) that produces 50% optimal binding response is found to be approximately 5-40 ng/mL.
Recombinant Human VSIG4 Fc Chimera Avi-tag Protein SDS-PAGE

Recombinant Human VSIG4 Fc Chimera Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human VSIG4 Fc Chimera Avi-tag (Catalog # AVI4646) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-85 kDa and 130-170 kDa.

Formulation, Preparation and Storage

AVI4646
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG4

V-set and immunoglobulin domain containing 4 (VSIG4), also known as CRIg and Z39IG, is a type I transmembrane protein of the B7 family and complement receptor of the immunoglobulin superfamily. Human VSIG4 consists of an extracellular domain (ECD) containing a V-type and a C2-type Ig domain, a transmembrane domain and a cytoplasmic domain (3). Interestingly, VISG4 in several animals, including mouse and rat, lack the C2-type Ig domain present in human VSIG4. Within the IgV domain, mature human VSIG4 shares 80% and 78% amino acid identity with mouse and rat VSIG4, respectively. Alternative splicing results in numerous isoforms lacking all or part of the cytoplasmic domain, the C2-type Ig domain and/or the transmembrane domain. VSIG4 is specifically expressed on macrophages in the thymic medulla, peritoneum, alveoli, synovia, adipose and heart, liver Kupffer cells, placental Hofbauer cells, and atherosclerotic foam cells (1-8). It is absent on infiltrating macrophages (8). VSIG4 is a complement receptor that binds C3b and iC3b fragments, internalizes them to recycling endosomes, and is recycled to the cell surface (4, 5). It contributes significantly to innate immunity by binding and phagocytosis of complement-opsonized invading pathogens (4, 7, 9). Binding of either native or recombinant soluble VSIG4 to C3b inhibits complement amplification through the alternative, but not classical, pathway (9, 10). VSIG4 is also a negative regulator of mouse and human T cell activation (2). Although VSIG4 engagement may activate NF kappaB and thus be pro-inflammatory in some cases, many of its activities are important in resolving, rather than initiating, inflammation (1, 2, 6, 9, 10). VSIG4 expression has been implicated in lung cancer development and associated with poor prognosis of high grade glioma (11, 12). Our Avi-tag Biotinylated Recombinant VSIG4 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. 1. He, J.Q. et al. (2008) Mol. Immunol. 4041.
  2. Vogt, L. et al. (2006) J. Clin. Invest. 116:2817.
  3. Langnaese, K. et al. (2000) Biochim. Biophys. Acta 1492:522.
  4. Helmy, K. et al. (2006) Cell 124:915.
  5. Tanaka, M. et al. (2008) Clin. Exp. Immunol. 154:38.
  6. Lee, M-Y. et al. (2006) J. Leukoc. Biol. 80:922.
  7. Gorgani, N.N. et al. (2008) J. Immunol. 181:7902.
  8. Walker, M.G. (2002) Biochim. Biophys. Acta 1574:387.
  9. Wiesmann, C. et al. (2006) Nature 444:217.
  10. Katschke, K.J. et al. (2007) J. Exp. Med. 204:1319.

Long Name

V-Set and Immunoglobulin Domain Containing 4

Alternate Names

Z39IG

Entrez Gene IDs

11326 (Human); 278180 (Mouse); 312102 (Rat); 102144784 (Cynomolgus Monkey)

Gene Symbol

VSIG4

UniProt

Q9Y279.1

Additional VSIG4 Products

Product Documents for Recombinant Human VSIG4 Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human VSIG4 Fc Chimera Avi-tag Protein, CF

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