Recombinant Human XIAP (BIR2) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 786-XB
Key Product Details
Product Specifications
Source
E. coli-derived human XIAP protein
Ala124-Ala242, with an N-terminal Met and 6-His tag
Ala124-Ala242, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
15 kDa
SDS-PAGE
12 kDa, reducing conditions
Formulation, Preparation and Storage
786-XB
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: XIAP
XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits Caspase-3 and Caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.
SUPPLEMENTARY METHODS
XIAP (BIR2 domain) inhibits DEVD-AFC cleavage by activated cell extracts.
XIAP (BIR2 domain) inhibits DEVD-AFC cleavage by Recombinant Human Caspase-3
Recombinant Human Caspase‑3 (Catalog # http://www.rndsystems.com/product_results.aspx?k=707-C3">707-C3) can be used in the Activity Assay Protocol in place of Casapase-7 by diluting to initial concentration of 32 pg/uL in Step 2.
The IC50 for inhibition of rhCaspase-3 activity is typically 10-100 nM.
References
- Deveraux, Q. et al. (1998) EMBO J. 17(8):2215.
- Deveraux, Q. et al. (1999) EMBO J. 18(19):5242.
- Verhagen, A. et al. (2000) Cell 102:43.
- Chai, J. et al. (2001) Cell 104:769.
- Riedl, S. (2001) Cell 104:791.
- Suzuki, Y. (2001) J. Biol. Chem. 276(29):27058.
- Ekert, P. et al. (2001) J. Cell Biol. 152(3):483.
- Du, C. et al. (2000) Cell 102:33.
Long Name
X-linked Inhibitor of Apoptosis
Alternate Names
BIRC4
Gene Symbol
XIAP
UniProt
Additional XIAP Products
Product Documents for Recombinant Human XIAP (BIR2) Protein, CF
Product Specific Notices for Recombinant Human XIAP (BIR2) Protein, CF
For research use only
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