Recombinant Mouse Angiopoietin-1 Protein

R&D Systems, part of Bio-Techne | Catalog # 9936-AN

R&D Systems, part of Bio-Techne
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Carrier Free
9936-AN-025/CF
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With Carrier
9936-AN-025
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Key Product Details

Source

NS0

Accession #

O08538

Structure / Form

Disulfide-linked Homooligomer

Conjugate

Unconjugated

Applications

Bioactivity

View all Angiopoietin-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Angiopoietin-1 protein
Mouse Angiopoietin-1
(Ser20-Leu261)
Accession # O08538		 GGGSGGGSGGGS Mouse Angiopoietin-1
(Arg277-Phe498)
Accession # O08538		 HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

56 kDa

SDS-PAGE

59-75 kDa, reducing conditions

Activity

Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J. et al. (1999) FEBS Letters 448:249.
The ED50 for this effect is 10-50 ng/mL in the presence of 5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Scientific Data Images for Recombinant Mouse Angiopoietin-1 Protein

Recombinant Mouse Angiopoietin-1 Protein Bioactivity

Recombinant Mouse Angiopoietin-1 Protein Bioactivity

Recombinant Mouse Angiopoietin-1 inhibits serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 10-50 ng/mL in the presence of 5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Recombinant Mouse Angiopoietin-1 Protein SDS-PAGE

Recombinant Mouse Angiopoietin-1 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Angiopoietin‑1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 59-75 kDa and oligomer, respectively.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9936-AN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Carrier Free: 9936-AN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system (1, 2). It contains a N-terminal coiled-coil region and a C-terminal fibrinogen-like domain separated by a short flexible region (3, 4). Mature Mouse Angiopoietin-1 shares 97% and 98% amino acid sequence identity with Human and rat Angiopoietin-1, respectively. It is expressed by vascular smooth muscle cells and pericytes as an approximately 70 kDa molecule that associates into disulfide-linked homotrimers, tetramers, and pentamers (3, 5). Angiopoietin-1 binds and activates the receptor tyrosine kinase Tie-2, and its association into tetramers is important for full Tie-2 activation (3, 4). Angiopoietin-1 ligation of Tie-2 on vascular endothelial cells (EC) induces the development and branching of blood vessels (6, 7). In sub-confluent EC (i.e. during angiogenesis), Angiopoietin-1 promotes EC motility and Tie-2 localization at the trailing edge of the cell (8). In confluent EC (i.e. in homeostasis), multimeric Angiopoietin-1 enhances vascular integrity by promoting the in trans homotypic association of Tie-2 between EC or with the substratum (8, 9). In addition, Angiopoietin-1 suppresses several VEGF-induced effects on the vasculature including endothelial permeability, stretch-induced release of Angiopoietin-2, and up-regulation of the leukocyte adhesion molecules VCAM-1, ICAM-1, and E-Selectin (10-12). Angiopoietin-1 also interacts with a variety of integrins and the extracellular matrix independently of Tie-2 (13, 14). These interactions support the adhesion, migration and stress resistance of EC, fibroblasts, and myocytes (13, 14). Angiopoietin-1 can protect against pulmonary arterial hypertension (5), reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium (15), and enhance tumor progression and metastasis (16).

References

  1. Koh, G.Y. (2012) Trends Mol. Med. 19:31.
  2. Suri, C. et al. (1996) Cell 87:1171.
  3. Davis, S. et al. (1996) Cell 87:1161.
  4. Kim, K.-T. et al. (2005) J. Biol. Chem. 280:20126.
  5. Kugathasan, L. et al. (2009) J. Exp. Med. 206:2221.
  6. Suri, C. et al. (1998) Science 282:468.
  7. Jeansson, M. et al. (2011) J. Clin. Invest. 121:2278.
  8. Saharinen, P. et al. (2008) Nat. Cell. Biol. 10:527.
  9. Fukuhara, S. et al. (2008) Nat. Cell Biol. 10:513.
  10. Jho, D. et al. (2005) Circ. Res. 96:1282.
  11. Korff, T. et al. (2012) Cardiovasc. Res. 94:510.
  12. Kim, I. et al. (2001) Circ. Res. 89:477.
  13. Carlson, T.R. et al. (2001) J. Biol. Chem. 276:26516.
  14. Dallabrida, S.M. et al. (2005) Cardiovasc. Res. 96:e8.
  15. Samuel, S.M. et al. (2010) Diabetes 59:51.
  16. Holopainen, T. et al. (2009) Cancer Res. 69:4656.

Alternate Names

ANGPT1

Entrez Gene IDs

284 (Human); 11600 (Mouse)

Gene Symbol

ANGPT1

UniProt

O08538

Additional Angiopoietin-1 Products

Product Documents for Recombinant Mouse Angiopoietin-1 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 9936-AN (with carrier)
Download SDS 9936-AN/CF (carrier free)

Product Specific Notices for Recombinant Mouse Angiopoietin-1 Protein

For research use only

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