Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9899-AN

R&D Systems, part of Bio-Techne
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9899-AN-050
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Key Product Details

Source

NS0

Accession #

Q9R182

Conjugate

Unconjugated

Applications

Bioactivity

View all Angiopoietin-like Protein 3/ANGPTL3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 3/ANGPTL3 protein
Hemagglutinin Tag
(YPYDVPDYA)
 Mouse Angiopoietin-like Protein 3
(Ser17-Thr455)
Accession # Q9R182
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

52 kDa

SDS-PAGE

59-71 kDa and 33-38 kDa, reducing conditions

Activity

Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770.
The IC50 value under conditions in which Recombinant Human Lipoprotein Lipase  (Catalog # 9888-LL) is present at 0.5 μg/mL, and p‑nitrophenyl butyrate concentration is 1 mM in 0.1 M sodium phosphate, 0.15 M NaCl, 0.5% Triton® X-100, pH 7.2, is 2-10 µg/mL.

Scientific Data Images for Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein, CF

Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein Bioactivity

Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein Bioactivity

Recombinant Mouse ANGPTL3 (Catalog # 9899-AN) dose dependently inhibits Recombinant Human LPL (Catalog # 9888-LL) activity with an IC50of 2-10 µg/mL.
Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein SDS-PAGE

Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Angiopoietin‑like Protein 3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 59 - 71 kDa and 33 - 38 kDa.

Formulation, Preparation and Storage

9899-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS with Trehalose.
Reconstitution
Reconstitute at 400 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins (1-3). Mature mouse ANGPTL3 contains an N-terminal coiled‑coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C‑terminal fragments containing the coiled‑coil domain and fibrinogen-like domains, respectively (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL), an enzyme responsible for hydrolyzing circulating triglycerides (8). This activity requires a putative heparin-binding motif that is N-terminal to the coiled-coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6-8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (9). ANGPTL3 expression in vivo is up-regulated by LXR agonists and down-regulated by insulin, leptin, and TR beta agonists (10-12). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice, (7, 8, 11). ANGPTL3 does not bind Tie-1 or Tie-2 but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (13). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (14). Mature mouse ANGPTL3 shares 22% - 30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 6, and 7. It shares 77% aa sequence identity with human ANGPTL3.

References

  1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
  2. Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
  3. Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.
  4. Conklin, D. et al. (1999) Genomics 62:477.
  5. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  6. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
  7. Koishi, R. et al. (2002) Nat. Genet. 30:151.
  8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
  9. Koster, A. et al. (2005) Endocrinology 146:4943.
  10. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
  11. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
  12. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
  13. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
  14. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

UniProt

Q9R182 (Mouse)

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein, CF

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