Recombinant Mouse ASGR2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10447-AS

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

P24721.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ASGR2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse ASGR2 protein
MDP Mouse IgG2A
(Glu98-Lys330)		 IEGR Mouse ASGR2
(Ser79-His301)
Accession # P24721.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

53 kDa

SDS-PAGE

62-81 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse ASGR2 Fc Chimera (Catalog # 10447-AS) binds Human plasma von Willebrand Factor with an ED50 of 0.05-0.6 μg/mL.

Scientific Data Images for Recombinant Mouse ASGR2 Fc Chimera Protein, CF

Recombinant Mouse ASGR2 Fc Chimera Protein Bioactivity

Recombinant Mouse ASGR2 Fc Chimera Protein Bioactivity

Immobilized Recombinant Mouse ASGR2 Fc Chimera (10447-AS) binds human plasma von Willebrand Factor with an ED50 of 0.05-0.6 μg/mL.
Recombinant Mouse ASGR2 Fc Chimera Protein SDS-PAGE

Recombinant Mouse ASGR2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse ASGR2 Fc Chimera (Catalog # 10447-AS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-81 kDa and 120-160 kDa, respectively.

Formulation, Preparation and Storage

10447-AS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASGR2

Asialoglycoprotein receptor 2 (ASGR2), also known as hepatic lectin 2 (HL-2), is an approximately 38 kDa member of the C-type lectin receptor family (1). ASGR2 is a single-pass type II transmembrane protein that plays a role in the endocytosis of desialylated glycoproteins, hepatic thrombopoietin production, and gastrointestinal malignancies (2, 3). ASGR2 is closely related to ASGR1 which is about 2 kDa smaller and is encoded by distinct but closely linked genes. Variations in ASGR2 structure due to alternative splicing have been reported (4-6). Mature ASGR2 consists of a cytoplasmic domain, transmembrane segment and an extracellular domain (ECD). Within the ECD, mouse ASGR2 shares 67% and 83% aa sequence identity with human and rat ASGR2, respectively. Both ASGR2 and ASGR1 compose the asialoglycoprotein receptor (ASGPR) which mediates removal of potentially hazardous glycoconjugates from blood in health and disease (7). ASGPR, also known as the Ashwell receptor, can modulate von Willebrand factor (vWF) and platelet homeostasis in part via clearance of platelets that are first desialylated during sepsis caused by pathogens including S. pneumoniae. The ASGR1 chain of the Ashwell receptor participates in plasma vWF clearance independently of sialylation and sepsis, ASGR2 colocalization with plasma vWF was increased in ASGR1 deficient mice (8). In addition, ASGR2 expression was shown to be associated with malignant phenotypes in gastric cancer and may represent a specific biomarker for recurrence of some gastric cancers (9).

References

  1. Ishibashi, S. et al. (1994) J. Biol. Chem. 269:27803.
  2. Grozovsky, R. et al. (2015) Nat Med. 1:47.
  3. Tanaka, H. et al. (2018) Mol. Cancer Res. 16:1420.
  4. Drickamer, K. et al. (1984) J. Biol. Chem. 259:770.
  5. Halberg, D.F. et al. (1987) J. Biol. Chem. 262:9828.
  6. Paietta, E. et al. (1992) J. Biol. Chem. 267:11078.
  7. Harris R.L. et al. (2012) Mol Biol Int. 2012:283974.
  8. Grewal, P. et al. (2008) Nature Medicine. 14:6.
  9. Tanaka H. et al. (2018) Mol Cancer Res. 16:1420.

Long Name

Asialoglycoprotein Receptor 2

Alternate Names

ASGPR2, CLEC4H2, HBXBP

Entrez Gene IDs

433 (Human); 11890 (Mouse); 29403 (Rat)

Gene Symbol

ASGR2

UniProt

P24721.1

Additional ASGR2 Products

Product Documents for Recombinant Mouse ASGR2 Fc Chimera Protein, CF

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