Recombinant Mouse B7-2/CD86 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 741-B2

R&D Systems, part of Bio-Techne
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741-B2-100
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q549Q9

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all B7-2/CD86 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse B7-2/CD86 protein
Mouse B7-2
(Val26-Glu245)
Accession # Q549Q9		 GDIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val26

Predicted Molecular Mass

51.8 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909.
The ED50 for this effect is 0.05-0.15 µg/mL in the presence of PHA.

Formulation, Preparation and Storage

741-B2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-2/CD86

B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance (1, 2). Mature mouse B7-2 consists of a 221 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail (3, 4). Within the ECD, mouse B7-2 shares 59% and 81% aa sequence identity with human and rat B7-2, respectively. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells, and monocytes (4-7), as well as on vascular endothelial cells (8). B7-2 and the closely related B7-1/CD80 exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent co-stimulation (3-5, 7, 9-11). B7-1 and B7-2 additionally bind the CD28-related protein, CTLA-4, which is up‑regulated and recruited to the immunological synapse (IS) at the onset of T cell activation (3-5, 7, 9, 10). CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function (12). B7-2 is expressed earlier than B7-1 following APC activation (6), and both proteins bind with higher affinity to CTLA-4 than to CD28 (10). B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS (13). The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response (14). Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses (15).

References

  1. Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Chen, C. et al. (1994) J. Immunol. 152:4929.
  4. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  5. Azuma, M. et al. (1993) Nature 366:76.
  6. Lenschow, D.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11054.
  7. Hathcock, K.S. et al. (1993) Science 262:905.
  8. Seino, K. et al. (1995) Int. Immunol. 7:1331.
  9. Freeman, G.J. et al. (1993) Science 262:909.
  10. Lanier, L.L. et al. (1995) J. Immunol. 154:97.
  11. Rudd, C.E. et al. (2009) Immunol. Rev. 229:12.
  12. Wing, K. et al. (2011) Trends Immunol. 32:428.
  13. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
  14. Kuchroo, V.K. et al. (1995) Cell 80:707.
  15. Short, J.J. et al. (2006) Virus Res. 122:144.

Alternate Names

B72, CD86

Entrez Gene IDs

942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)

Gene Symbol

CD86

UniProt

Q549Q9

Additional B7-2/CD86 Products

Product Documents for Recombinant Mouse B7-2/CD86 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse B7-2/CD86 Fc Chimera Protein, CF

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