Recombinant Mouse B7-H2 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 158-B7
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse B7-H2 protein
| Mouse B7-H2 (Glu47 - Lys279) Accession # Q9JHJ8 |
DIEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu47
Predicted Molecular Mass
53 kDa (monomer)
SDS-PAGE
80-90 kDa, reducing conditions
Activity
Measured in a competitive binding assay.
When rmICOS/Fc Chimera (Catalog # 168-CS) is immobilized at 1 µg/mL (100 µL/well), rmB7-H2 inhibits 50% binding of biotinylated rhB7-H2/Fc Chimera (2.5 ng/mL) at the concentration range of 80-120 ng/mL.
When rmICOS/Fc Chimera (Catalog # 168-CS) is immobilized at 1 µg/mL (100 µL/well), rmB7-H2 inhibits 50% binding of biotinylated rhB7-H2/Fc Chimera (2.5 ng/mL) at the concentration range of 80-120 ng/mL.
Reviewed Applications
Read 1 review rated 5 using 158-B7 in the following applications:
Formulation, Preparation and Storage
158-B7
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: B7-H2
Mouse B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune co‑stimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. The extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Mouse and human B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of co‑stimulatory receptors. Mouse B7-H2 is a 322 amino acid (aa) protein with a putative 46 aa signal peptide, a 233 aa extracellular domain, a 19 aa transmembrane region, and a 24 aa cytoplasmic domain. Mouse B7-H2 is expressed constitutively on resting B cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
References
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Ling, V. et al. (2000) J. Immunol. 164:1653.
- Wang, S. et al. (2000) Blood 96:2808.
- Brodie, D. et al. (2000) Curr. Biol. 10:333.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Swallow, M.M. et al. (1999) Immunity 11:423.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
B7 Homolog 2
Alternate Names
B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG
Gene Symbol
ICOSLG
UniProt
Additional B7-H2 Products
Product Documents for Recombinant Mouse B7-H2 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse B7-H2 Fc Chimera Protein, CF
For research use only
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