Recombinant Mouse BID (Caspase-8-cleaved) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 883-M8

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
883-M8-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

P70444

Conjugate

Unconjugated

Applications

Bioactivity

View all BID Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse BID protein
Met1-Asp195

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

N-terminal Sequence Analysis

Met1 & Gly60

Predicted Molecular Mass

7 kDa (N-terminal fragment) & 15 kDa (C-terminal fragment)

SDS-PAGE

7 kDa and 15 kDa, under reducing and non-reducing conditions

Activity

Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.
The typical EC50 for cytochrome c releasing activity is <100 nM. Cytochrome c is quantified using the Rat/Mouse Cytochrome c Quantikine® ELISA Kit (Catalog # MCTC0).
The EC50 for the desired application should be determined.
Uncleaved Recombinant Mouse BID is available (Catalog # 860-MB).

Formulation, Preparation and Storage

883-M8
Formulation Supplied as a 0.2 μm filtered solution in HEPES-KOH and KCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: BID

Bid is a 195 amino acid member of the Bcl‑2 family of proteins that regulates outer mitochondrial membrane permeability (1). Bid is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells Bid is cytosolic. In response to Fas ligand or TNF Bid is cleaved by Caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of Bid by Caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target Bid to the mitochondria (4). Bid may then interact with another pro-apoptotic Bcl‑2 family member Bak (5). Interaction of Bid with Bak causes altered mitochondrial membrane permeability. A 9-13 amino acid stretch called the BH3 region (Bcl‑2 homology region) appears to mediate the Bid interaction with other Bcl‑2 family members. Bid is neutralized by binding to the anti-apoptotic member Bcl‑xL.

References

  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Luo, X., et al. (1998) Cell 94:481.
  3. Li, H. et al. (1998) Cell 94:491.
  4. Zha, J. et al. (2000) Science 290:1761.
  5. Wei, M.C. et al. (2000) Genes Dev. 14:2060.
 

Long Name

BH3 Interacting Domain Death Agonist

Alternate Names

apoptic death agonist, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p22 BID, tbid

Entrez Gene IDs

637 (Human); 12122 (Mouse)

Gene Symbol

BID

UniProt

P70444

Additional BID Products

Product Documents for Recombinant Mouse BID (Caspase-8-cleaved) Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse BID (Caspase-8-cleaved) Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.