Recombinant Mouse CDO His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10505-CD

R&D Systems, part of Bio-Techne
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10505-CD-050
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Key Product Details

Source

NS0

Accession #

Q32MD9.2

Conjugate

Unconjugated

Applications

Bioactivity

View all CDO Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CDO protein
Asp25-Tyr962, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp25

Predicted Molecular Mass

103 kDa

SDS-PAGE

113-135 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Sonic Hedgehog/Shh (C25II), N-Terminus  (Catalog # 464-SH) is immobilized at 4 µg/mL (100 µL/well), Recombinant Mouse CDO His-tag (Catalog # 10505-CD) binds with an ED50 of 0.8-8 ng/mL.

Scientific Data Images for Recombinant Mouse CDO His-tag Protein, CF

Recombinant Mouse CDO His-tag Protein Binding Activity.

When Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus (464-SH) is immobilized at 4 µg/mL (100 µL/well), Recombinant Mouse CDO His-tag (Catalog # 10505-CD) binds with an ED50 of 0.8-8 ng/mL.

Recombinant Mouse CDO His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse CDO His-tag Protein (10505-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 113-135 kDa.

Formulation, Preparation and Storage

10505-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDO

CAM-related/down-regulated by oncogene (CDO), also known as CDON is a member of the Ig/Fibronectin (FN) type III repeat family within the Ig superfamily. Mouse CDO is a type I transmembrane protein, consisting of a large extracellular domain (ECD), a transmembrane segment and a cytoplasmic region. The ECD contains five C2-type Ig-like domains, followed by three FN type III repeats (1). The first FN repeat is known to bind numerous cadherins, while the third (or juxtramembrane) FN type III repeat binds SHH (2, 3). The mature ECD of mouse CDO shares 85% amino acid identity to the ECD of human CDO. CDO is found on muscle precursor and neural progenitor cells of the embryo (4-6). It likely promotes muscle differentiation and contributes to axon guidance and neuronal patterning by signaling through various bHLH transcription factors (2, 5). These effects may be mediated through two different receptor complexes. On muscle precursors, CDO apparently acts as both a coordinating and signaling subunit. Here, it integrates N- and M-cadherin, neogenin, netrin-3 and BOC into a cis-oriented receptor complex (4, 7). While this complex has no identified ligand, intercellular cadherin interactions or netrin, may be enough to trigger CDO/cadherin/neogenin signaling. On axons, CDO may participate in a poorly-defined receptor complex minimally composed of CDO, BOC and Gas1 that binds SHH, and interacts with PTCH1 (8-10).

References

  1. Kang, J.S. et al. (1997) J. Cell Biol. 138:203.
  2. Yao, S. et al. (2006) Cell 125:343.
  3. Kang, J-S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3989.
  4. Kang, J-S. et al. (2002) EMBO J. 21:114.
  5. Zhang, W. et al. (2006) Mol. Cell. Biol. 26:3764.
  6. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355.
  7. Kang, J-S. et al. (2004) J. Cell. Biol. 167:493.
  8. Okada, A. et al. (2006) Nature 444:369.
  9. Allen, B.L. et al. (2007) Genes Dev. 21:1244.
  10. Tenzen, T. et al. (2006) Dev. Cell 10:647.

Long Name

Cell Adhesion Molecule-related/Down-regulated by Oncogenes

Alternate Names

CDON, ORCAM

Entrez Gene IDs

50937 (Human); 57810 (Mouse)

Gene Symbol

CDON

UniProt

Q32MD9.2

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