Recombinant Mouse Coagulation Factor XIV/Protein C, CF
R&D Systems, part of Bio-Techne | Catalog # 4885-SE
Key Product Details
Source
CHO
Accession #
Structure / Form
Pro and Mature forms
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Coagulation Factor XIV/Protein C protein
Ile19-Leu460 (pro) & Ala42-Leu460 (mature), both with a C-terminal 10-His tag
Ile19-Leu460 (pro) & Ala42-Leu460 (mature), both with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile19 & Ala42
Predicted Molecular Mass
49 kDa (Pro) & 48 kDa (Mature)
SDS-PAGE
70 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Boc-beta -benzyl-DPR-AMC.
The specific activity is >125 pmol/min/µg, as measured under the described conditions.
The specific activity is >125 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4885-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Coagulation Factor XIV/Protein C
Protein C is a vitamin K-dependent serine protease synthesized in the liver as a single-chain precursor, which is then proteolytically processed to two disulfide-linked chains (1). The light chain consists of a Gla (gamma-carboxy-glutamate) domain and two EGF-like domains. The heavy chain consists of an activation peptide (aa 199‑212) and serine protease domain (aa 213‑449). Physiologically, Protein C is converted to the active form by thrombin, which releases the activation peptide. Protein C plays a key role in anticoagulation, cleaving factors VIIIa and Va to inactivate them. This anticoagulation activity can be enhanced by a presence of a cofactor such as protein S. In hereditary thrombophilia, Protein C deficiency is caused by a genetic mutation that affects Protein C activity. A severe recessive form may result in massive thrombosis fatal to patient.
References
- Shen, L. and Dahlbäck, B. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1673.
Long Name
Vitamin K-dependent Protein C
Alternate Names
Autoprothrombin IIA, PROC, Protein C
Gene Symbol
PROC
UniProt
Additional Coagulation Factor XIV/Protein C Products
Product Documents for Recombinant Mouse Coagulation Factor XIV/Protein C, CF
Product Specific Notices for Recombinant Mouse Coagulation Factor XIV/Protein C, CF
For research use only
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