Recombinant Mouse Complement Factor H Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4999-FH

R&D Systems, part of Bio-Techne
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4999-FH-050
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Key Product Details

Source

NS0

Accession #

NP_034018

Conjugate

Unconjugated

Applications

Bioactivity

View all Complement Factor H Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Complement Factor H protein
Ser875-Val1252, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser875

Predicted Molecular Mass

43.2 kDa

SDS-PAGE

64 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to induce the adhesion of human neutrophils. DiScipio, R.G. et al. (1998) J. Immunol. 160:4057.

The ED50 for this effect is 3-18 μg/mL in the presence of 50 ng/mL of rhTNF-alpha.

Formulation, Preparation and Storage

4999-FH
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. 
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H

Factor H is a 155 kDa glycoprotein that provides critical negative regulation of the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration (1). Factor H is composed of 20 SCR (short consensus repeats), each of which consists of approximately 60 amino acids with four invariant Cys residues (2). Factor H interacts with cell surface polyanions including heparin and sialoglycoproteins (3 - 6), and immobilized Factor H supports the CD11b/CD18 integrin-dependent adhesion of neutrophils (7). It prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5 convertases, and functioning as a cofactor for the C3b inactivator, Factor I (1, 3, 6, 8). This recombinant protein corresponds to SCR15-20 which encompass the primary binding sites for heparin and C3b as well as for the peptide hormone adrenomedullin (4, 9 - 11). Within SCR15-20, mouse Factor H shares 60% and 80% amino acid sequence identity with human and rat Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure (12). Binding of Factor H to tumor cell-associated dentin matrix protein 1, bone sialoprotein, or osteopontin results in the protection of that cell from complement mediated lysis (13, 14). A variety of pathogenic microbes also express Factor H binding molecules that interfere with immune clearance of the infection (15).

References

  1. Schmidt, C.Q. et al. (2008) Clin. Exp. Immunol. 151:14.
  2. Kristensen, T. and B.F. Tack (1986) Proc. Natl. Acad. Sci. 83:3963.
  3. Meri, S. and M.K. Pangburn (1990) Proc. Natl. Acad. Sci. 87:3982.
  4. Jokiranta, T.S. et al. (2005) Am. J. Pathol. 167:1173.
  5. Blackmore, T.K. et al. (1998) J. Immunol. 160:3342.
  6. Hellwage, J. et al. (2002) J. Immunol. 169:6935.
  7. DiScipio, R.G. et al. (1998) J. Immunol. 160:4057.
  8. Sharma, A.K. and M.K. Pangburn (1996) Proc. Natl. Acad. Sci. 93:10996.
  9. Oppermann, M. et al. (2006) Clin. Exp. Immunol. 144:342.
  10. Pangburn, M.K. et al. (2000) J. Immunol. 164:4742.
  11. Martinez, A. et al. (2003) Hypertens. Res. 26:S55.
  12. de Cordoba, S.R. and E.G. de Jorge (2008) Clin. Exp. Immunol. 151:1.
  13. Jain, A. et al. (2002) J. Biol. Chem. 277:13700.
  14. Fedarko, N.S. et al. (2000) J. Biol. Chem. 275:16666.
  15. Kraiczy, P. and R. Wurzner (2006) Mol. Immunol. 43:31.

Alternate Names

ARMD4, ARMS1, beta-1-H-globulin, CFH, CFHL3, FH, FHL1, HF1, HF2, HUS

Entrez Gene IDs

3075 (Human); 12628 (Mouse); 155012 (Rat)

Gene Symbol

CFH

UniProt

NP_034018

Additional Complement Factor H Products

Product Documents for Recombinant Mouse Complement Factor H Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Complement Factor H Protein, CF

For research use only

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